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Genetics and Molecular Biology |
Institute of Food Research, Norwich Research Park, Colney Lane, NR4 7UA Norwich, UK1
Author for correspondence: Annette Griffin. Tel: +44 1603 255 354. Fax: +44 1603 507 723. e-mail: annette.griffin{at}bbsrc.ac.uk
The cpsFGHIJKL genes from the cps cluster of Streptococcus thermophilus NCFB 2393 involved in the biosynthesis of EPS were identified, cloned and nucleotide sequenced. The complete cps cluster is contained on an 
11·2 kb chromosomal region which contains 12 ORFs, including the previously cloned cpsABCDE genes. Functions were assigned to some of the predicted gene products on the basis of homology to known sequences as follows: cpsK encodes a protein thought to be involved in the polymerization and export of the polysaccharide; cpsE, cpsF, cpsG, cpsH, cpsI and cpsJ encode putative sugar transferases. Two insertion sequences, IS1193 and ISS1, were identified within and flanking the 3' end of the cps cluster respectively. Analysis of the expression of the cpsE gene in Escherichia coli demonstrated that it encodes a glucose-1-phosphate transferase; the enzyme which catalyses the first step in EPS biosynthesis in S. thermophilus NCFB 2393.
Keywords: capsule polysaccharide, exopolysaccharide, Streptococcus thermophilus, glycosyltransferase, IS1193
Abbreviations: CPS, capsule polysaccharide; EPS, exopolysaccharide
The GenBank accession number for the sequence reported in this paper is Y17900.
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