Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 146 (2000), 2877-2889
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huckauf, J.
Right arrow Articles by Hagemann, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huckauf, J.
Right arrow Articles by Hagemann, M.
Agricola
Right arrow Articles by Huckauf, J.
Right arrow Articles by Hagemann, M.
Microbiology (2000), 146, 2877-2889.
© 2000 Society for General Microbiology

Genetics and Molecular Biology

Stress responses of Synechocystis sp. strain PCC 6803 mutants impaired in genes encoding putative alternative sigma factors

Jana Huckauf1, Chris Nomura2, Karl Forchhammer3 and Martin Hagemann1

Universität Rostock, FB Biologie, Institut für Molekulare Physiologie und Biotechnologie, Doberaner Str. 143,D-18051 Rostock, Germany1
Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA2
Justus-Liebig-Universität Giessen, Institut für Mikrobiologie und Molekularbiologie, Frankfurter Str. 107,D-35392 Giessen, Germany3

Author for correspondence: Martin Hagemann. Tel: +49 381 4942076. Fax: +49 381 4942079. e-mail: mh{at}bio4.uni-rostock.de

In the complete genome sequence of the cyanobacterium Synechocystis sp. strain PCC 6803 [Kaneko et al. (1996 ). DNA Res 3, 109–136] genes were identified encoding putative group 3 {sigma}-factors SigH (Sll-0856), SigG (Slr-1545) and SigF (Slr-1564) and the regulatory protein RsbU (Slr-2031). Mutations in these genes were generated by interposon mutagenesis to study their importance in stress acclimation. For the genes sigH, sigF and rsbU, the loci segregated completely. However, attempts to mutagenize the sigG locus resulted in merodiploids. Under standard growth conditions only minor differences were detected between the mutants and wild-type. However, cells of the RsbU mutant showed a clear defect in regenerating growth after a nitrogen- and sulphur-starvation-induced stationary phase. After applying salt, heat and high-light shocks, stress protein synthesis was analysed by means of one- and two-dimensional electrophoresis. Cells of the SigF mutant showed a severe defect in the induction of salt stress proteins. Although the acclimation to moderate salt stress up to 684 mM NaCl was not significantly changed in this mutant, its ability to acclimate to higher concentrations of NaCl was reduced. Northern blot experiments showed a constitutive expression of the rsbU and sigF genes. The expression of the sigH gene was found to be stress-stimulated, particularly in heat-shocked cells, whilst that of sigG was transiently decreased under stress conditions. Possible functions of these regulatory proteins in stress acclimation of Synechocystis cells are discussed.

Keywords: cyanobacteria, environmental stress, sigma factors, stationary phase, stress proteins

Abbreviations: 1D, one-dimensional; 2D, two-dimensional; Km, kanamycin; PSII, photosystem II; WT, wild-type




This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
M. Asayama and S. Imamura
Stringent promoter recognition and autoregulation by the group 3 {sigma}-factor SigF in the cyanobacterium Synechocystis sp. strain PCC 6803
Nucleic Acids Res., September 1, 2008; 36(16): 5297 - 5305.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K. Asai, K. Ishiwata, K. Matsuzaki, and Y. Sadaie
A Viable Bacillus subtilis Strain without Functional Extracytoplasmic Function Sigma Genes
J. Bacteriol., April 1, 2008; 190(7): 2633 - 2636.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Sakuragi, H. Maeda, D. DellaPenna, and D. A. Bryant
{alpha}-Tocopherol Plays a Role in Photosynthesis and Macronutrient Homeostasis of the Cyanobacterium Synechocystis sp. PCC 6803 That Is Independent of Its Antioxidant Function
Plant Physiology, June 1, 2006; 141(2): 508 - 521.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
N. Murata and I. Suzuki
Exploitation of genomic sequences in a systematic analysis to access how cyanobacteria sense environmental stress
J. Exp. Bot., January 1, 2006; 57(2): 235 - 247.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
R. Li, M. B. Potters, L. Shi, and P. J. Kennelly
The Protein Phosphatases of Synechocystis sp. Strain PCC 6803: Open Reading Frames sll1033 and sll1387 Encode Enzymes That Exhibit both Protein-Serine and Protein-Tyrosine Phosphatase Activity In Vitro
J. Bacteriol., September 1, 2005; 187(17): 5877 - 5884.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
R. Schwarz and K. Forchhammer
Acclimation of unicellular cyanobacteria to macronutrient deficiency: emergence of a complex network of cellular responses
Microbiology, August 1, 2005; 151(8): 2503 - 2514.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Marin, Y. Kanesaki, D. A. Los, N. Murata, I. Suzuki, and M. Hagemann
Gene Expression Profiling Reflects Physiological Processes in Salt Acclimation of Synechocystis sp. Strain PCC 6803
Plant Physiology, October 1, 2004; 136(2): 3290 - 3300.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. Nagashima, M. Hanaoka, T. Shikanai, M. Fujiwara, K. Kanamaru, H. Takahashi, and K. Tanaka
The Multiple-Stress Responsive Plastid Sigma Factor, SIG5, Directs Activation of the psbD Blue Light-Responsive Promoter (BLRP) in Arabidopsis thaliana
Plant Cell Physiol., April 15, 2004; 45(4): 357 - 368.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Marin, I. Suzuki, K. Yamaguchi, K. Ribbeck, H. Yamamoto, Y. Kanesaki, M. Hagemann, and N. Murata
Identification of histidine kinases that act as sensors in the perception of salt stress in Synechocystis sp. PCC 6803
PNAS, July 22, 2003; 100(15): 9061 - 9066.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
I. Tuominen, E. Tyystjarvi, and T. Tyystjarvi
Expression of Primary Sigma Factor (PSF) and PSF-Like Sigma Factors in the Cyanobacterium Synechocystis sp. Strain PCC 6803
J. Bacteriol., February 1, 2003; 185(3): 1116 - 1119.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K. Marin, J. Huckauf, S. Fulda, and M. Hagemann
Salt-Dependent Expression of Glucosylglycerol-Phosphate Synthase, Involved in Osmolyte Synthesis in the Cyanobacterium Synechocystis sp. Strain PCC 6803
J. Bacteriol., June 1, 2002; 184(11): 2870 - 2877.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Irmler and K. Forchhammer
A PP2C-type phosphatase dephosphorylates the PII signaling protein in the cyanobacterium Synechocystis PCC 6803
PNAS, October 25, 2001; (2001) 231254998.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Irmler and K. Forchhammer
A PP2C-type phosphatase dephosphorylates the PII signaling protein in the cyanobacterium Synechocystis PCC 6803
PNAS, November 6, 2001; 98(23): 12978 - 12983.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2000 Society for General Microbiology.