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Catabolite repression of dra–nupC–pdp operon expression in Bacillus subtilis

Department of Microbiology, Technical University of Denmark, Building 301, DK-2800 Lyngby, Denmark¹
Institut de Biologie et Chimie des Protéines, CNRS, 7 Passage du Vercors, F-69376 Lyon, Cedex 07, France²

Author for correspondence: Hans H. Saxild. Tel: +45 25 24 95. Fax: +45 88 26 60. e-mail: imhhs{at}pop.dtu.dk

Expression of the Bacillus subtilis dra–nupC–pdp operon is subject to catabolite repression by glucose. It was shown that a cis-acting catabolite-responsive element (CRE) sequence located 64 bp downstream of the transcription-start site mediated catabolite repression of the dra–nupC–pdp operon as it does for many other B. subtilis genes. Point mutations in the CRE sequence caused the loss of catabolite repression of the operon. Catabolite repression of dra–nupC–pdp expression was relieved in a ccpA mutant and was found to be dependent on both HPr and the HPr-like protein Crh. Furthermore, a transcription-repair coupling factor, Mfd, was also found to be involved in the glucose repression of dra–nupC–pdp expression. By the use of in vitro gel mobility shift analysis, a specific HPr-P dependent binding of CcpA to the dra CRE site was demonstrated.

Keywords: Glucose catabolite repression, nucleoside catabolism, dra–nupC–pdp operon, Bacillus subtilis, CRE

Abbreviations: CRE, catabolite-responsive element; O_deoR, DeoR operator

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