Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 146 (2000), 2929-2936
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rivas, L. A.
Right arrow Articles by Mellado, R. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rivas, L. A.
Right arrow Articles by Mellado, R. P.
Agricola
Right arrow Articles by Rivas, L. A.
Right arrow Articles by Mellado, R. P.
Microbiology (2000), 146, 2929-2936.
© 2000 Society for General Microbiology

Genetics and Molecular Biology

The Bacillus subtilis 168 csn gene encodes a chitosanase with similar properties to a Streptomyces enzyme

Luis A. Rivas1, Víctor Parro1, Mercedes Moreno-Paz1 and Rafael P. Mellado1

Centro Nacional de Biotecnología (CSIC), Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain1

Author for correspondence: Rafael P. Mellado. Tel: +34 91 5854547. Fax: +34 91 5854506. e-mail: rpmellado{at}cnb.uam.es

The Bacillus subtilis 168 csn gene encodes a chitosanase. It was found that transcription of the csn gene was temporally regulated and was not subject to metabolic repression. Chitosanase synthesis was abolished in a csn mutant strain. Csn was overproduced in B. subtilis, partially purified and characterized. The deduced amino acid sequence, Km, and optimal pH and temperature of the B. subtilis enzyme were closer to those of a chitosanase from Streptomyces sp. N174 than to those of chitosanases from other Bacillus strains.

Keywords: chitosanase, Bacillus subtilis, gene expression, catabolite repression

Abbreviations: LR-PCR, long-range PCR




This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
C. Yun, D. Amakata, Y. Matsuo, H. Matsuda, and M. Kawamukai
New Chitosan-Degrading Strains That Produce Chitosanases Similar to ChoA of Mitsuaria chitosanitabida
Appl. Envir. Microbiol., September 1, 2005; 71(9): 5138 - 5144.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2000 Society for General Microbiology.