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A flagellar gene cluster from the oral spirochaete Treponema maltophilum

Institut für Mikrobiologie und Hygiene¹ and Institut für Pharmakologie und Toxikologie², Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Dorotheenstr. 96, 10117 Berlin, Germany

Author for correspondence: Ulf B. Göbel. Tel: +49 3020934715. Fax: +49 3020934703. e-mail: ulf.goebel{at}charite.de

A flagellar gene cluster from the oral spirochaete Treponema maltophilum ATCC 51939^T was cloned. Sequence analysis revealed six putative ORFs, two of which encode the flagellar subunit proteins FlaB2 (286 aa) and FlaB3 (285 aa). Northern blot analysis revealed two flagellin transcripts with the expected size of monocistronic mRNAs. Sequence analysis and primer extension experiments indicated that the transcription of the flaB2 gene is directed by a ²⁸-like FliA factor. Using fliA and fliA⁺ Escherichia coli K-12 strains, it was shown that flaB2 expression in E. coli required the ²⁸ factor using an initiation site identical to that in Treponema maltophilum. Primer extension analysis revealed two transcriptional start sites 5' of the flaB3 gene, a strong promoter with a ²⁸-like -10 promoter element and a weak promoter with a putative ⁵⁴ promoter consensus sequence. Downstream of flaB3, a putative fliD homologue was found, probably encoding the flagellar cap protein of Treponema maltophilum. Flagellin-gene-specific DNA probes hybridized to all 13 Treponema strains investigated, whereas a fliD-specific DNA probe only hybridized to Treponema maltophilum, other treponemal group IV isolates and Treponema brennaborense.

Keywords: treponemes, Treponema maltophilum, flagellar filament, fliD

Abbreviations: CBP, calmodulin-binding protein; IP, isoelectric point; UAS, upstream activator sequence

The GenBank accession number for the sequence reported in this paper is Y18889.

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