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Microbiology 146 (2000), 741-747
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Microbiology (2000), 146, 741-747.
© 2000 Society for General Microbiology

Biochemistry

Proteins complexed to the P1 adhesin of Mycoplasma pneumoniae

Gerlinde Layh-Schmitta,1, Alexandre Podtelejnikovb,2 and Matthias Mannb,2

Hygiene-Institut, Universität Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany1
EMBL, Meyerhofstraße 1, 69123 Heidelberg, Germany2

Author for correspondence: Gerlinde Layh-Schmitt. Tel: +1 513 622 5509. Fax: +1 513 622 0085. e-mail: Layhschmitt.G{at}pg.com

Adherence of Mycoplasma pneumoniae to host cells requires several mycoplasmal membrane proteins and cytoskeleton-like proteins in addition to the adhesin P1, a transmembrane protein of 170 kDa. To analyse interactions of the P1 adhesin with other membrane proteins or with cytoskeleton-like proteins, cross-linking studies were performed in vivo using the permeant reagent paraformaldehyde. The cross-linked protein complex was isolated by immunoaffinity chromatography, and proteins complexed to the P1 protein were identified by immunoblot analysis followed by high mass accuracy tryptic peptide mapping using matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). In addition to the P1 protein and a truncated form of the same protein, the adhesin-related 30 kDa protein, two membrane proteins of 40 and 90 kDa, the cytoskeleton-associated 65 kDa protein and two cytoskeleton-forming proteins, HMW1 and HMW3, were found to be components of the isolated protein complex. Furthermore, the cross-linked complex contained the chaperone DnaK and the E1{alpha} subunit of pyruvate dehydrogenase. In summary, it was shown that cytadherence-associated membrane proteins are located in close proximity to cytoskeleton-like proteins, suggesting a functional interaction between membrane and cytoskeleton-like proteins. DnaK might be involved in translocation of proteins from the cytoplasm to the membrane and pyruvate dehydrogenase might be a structural protein of the attachment organelle.

Keywords: Mycoplasma pneumoniae, P1 adhesin, paraformaldehyde cross-linking

Abbreviations: MALDI MS, matrix-assisted laser desorption/ionization mass spectrometry

a Present address: Procter & Gamble Pharmaceuticals, Health Care Research Center, Anti-Infective Research, PO Box 8006, 8700 Mason-Montgomery Road, Mason, OH 45040, USA.

b Present address: Protein Interaction Laboratory, The University of Southern Denmark, Campusvej 55, DK 5230, Denmark.




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