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Altered patterns of cellular growth, morphology, replication and division in conditional-lethal mutants of the thermophilic archaeon Sulfolobus acidocaldarius

Department of Cell and Molecular Biology, Box 596, Biomedical Center, Uppsala University, SE-751 24 Uppsala, Sweden¹
Department of Biological Sciences, PO Box 210006, University of Cincinnati, Cincinnati, OH 45221-0006, USA²

Author for correspondence: Rolf Bernander. Tel: +46 18 471 40 58. Fax: +46 18 53 03 96. e-mail: Rolf.Bernander{at}icm.uu.se

As a basis for studing the essential cellular processes of hyperthermophilic archaea, thermosensitive mutants of Sulfolobus acidocaldarius were isolated and characterized. Exponential-phase liquid cultures were shifted to the non-permissive temperature and growth, viability, and distributions of cell mass and DNA content were measured as a function of time after the shift. The observed phenotypes demonstrate that chromosome replication, nucleoid organization, nucleoid partition and cell division, which normally are tightly co-ordinated during cellular growth, can be inhibited or uncoupled by mutation in this hyperthermophilic archaeon.

Keywords: Archaea, conditional mutants, flow cytometry, hyperthermophile, Sulfolobus

Abbreviations: DAPI, 4',6-diamidino-2-phenylindole; Ts, thermosensitive

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