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Disordered ultrastructure in lignin-peroxidase-secreting hyphae of the white-rot fungus Phanerochaete chrysosporium

School of Chemical and Life Sciences, University of Greenwich, Wellington Street, London SE18 6PF, UK¹
Department of Biology, Imperial College of Science Technology & Medicine, Prince Consort Road, London SW7 2BB, UK²

Author for correspondence: Patricia J. Harvey. Tel: +44 181 331 9972. Fax: +44 181 331 8305. e-mail: p.j.harvey{at}greenwich.ac.uk

The practice of exposing liquid cultures of the white-rot fungus Phanerochaete chrysosporium to a pure oxygen atmosphere under conditions of nutrient starvation has been widely adopted to induce lignin peroxidase (LiP) synthesis. Transmission electron microscopy was used to examine hyphal cells of carbon-limited cultures that had been exposed to an atmosphere of pure oxygen, and revealed evidence of a major loss in organization of cellular ultrastructure, which may be attributed to oxygen toxicity. Under some conditions (continuous agitation in air with cellulose as the carbon source) cultures will produce LiP without needing to be exposed to a pure oxygen atmosphere. A similar major loss of cellular ultrastructure was found in hyphal cells from such cultures upon examination. Investigation of the levels of H₂O₂, catalase and carbonyl content of intracellular proteins suggests that the latter cultures developed a hyperoxidant state because the rate of supply of carbon from cellulose hydrolysis was insufficient for oxygen homeostasis. The association of LiP with these cultures and with those exposed to an atmosphere of pure oxygen infers that LiP may be triggered in response to oxidant stress.

Keywords: cellulose, lignin peroxidase, Phanerochaete chrysosporium, oxidant stress, ultrastructure

Abbreviations: LiP, lignin peroxidase; NBT, nitro blue tetrazolium; TEM, transmission electron microscopy

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