HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bigi, F. Articles by Cataldi, A. Search for Related Content PubMed PubMed Citation Articles by Bigi, F. Articles by Cataldi, A. Agricola Articles by Bigi, F. Articles by Cataldi, A.

The gene encoding P27 lipoprotein and a putative antibiotic-resistance gene form an operon in Mycobacterium tuberculosis and Mycobacterium bovis

Instituto de Biotecnología¹ and Instituto de Patobiología², CICV-INTA, PO Box 77, Castelar, Argentina

Author for correspondence: A. Cataldi. Tel: +54 11 4621 0199. Fax: +54 11 4481 2975. e-mail: acataldi{at}inta.gov.ar

P27 is an antigenic membrane lipoprotein synthesized by members of the Mycobacterium tuberculosis complex. Northern blotting and RT-PCR experiments indicated that the genes encoding P27 and a putative antibiotic transporter (P55) form an operon. A promoter region was identified and characterized by deletion analysis in Mycobacterium smegmatis. Two transcription initiation points were mapped in Mycobacterium bovis BCG by primer extension analysis to 76 bp and 87 bp upstream of the ATG initiation codon. Putative -10 and -35 promoter consensus sequences associated with these showed 66% similarity to previously identified mycobacterial promoters. These results suggest that the P27/P55 operon is transcribed from two promoters in M. bovis BCG.

Keywords: Mycobacterium tuberculosis, Mycobacterium bovis, promoter, operon, lipoprotein

This article has been cited by other articles:

				M. F. Farrow and E. J. Rubin Function of a Mycobacterial Major Facilitator Superfamily Pump Requires a Membrane-Associated Lipoprotein J. Bacteriol., March 1, 2008; 190(5): 1783 - 1791. [Abstract] [Full Text] [PDF]

				P. Roback, J. Beard, D. Baumann, C. Gille, K. Henry, S. Krohn, H. Wiste, M.I. Voskuil, C. Rainville, and R. Rutherford A predicted operon map for Mycobacterium tuberculosis Nucleic Acids Res., August 1, 2007; 35(15): 5085 - 5095. [Abstract] [Full Text] [PDF]

				J. Turner, K. M. Dobos, M. A Keen, A. A. Frank, S. Ehlers, I. M. Orme, J. T. Belisle, and A. M. Cooper A Limited Antigen-Specific Cellular Response Is Sufficient for the Early Control of Mycobacterium tuberculosis in the Lung but Is Insufficient for Long-Term Survival Infect. Immun., July 1, 2004; 72(7): 3759 - 3768. [Abstract] [Full Text] [PDF]

				A.-H. Hovav, J. Mullerad, L. Davidovitch, Y. Fishman, F. Bigi, A. Cataldi, and H. Bercovier The Mycobacterium tuberculosis Recombinant 27-Kilodalton Lipoprotein Induces a Strong Th1-Type Immune Response Deleterious to Protection Infect. Immun., June 1, 2003; 71(6): 3146 - 3154. [Abstract] [Full Text] [PDF]

				P. E. A. Silva, F. Bigi, M. de la Paz Santangelo, M. I. Romano, C. Martín, A. Cataldi, and J. A. Aínsa Characterization of P55, a Multidrug Efflux Pump in Mycobacterium bovis and Mycobacterium tuberculosis Antimicrob. Agents Chemother., March 1, 2001; 45(3): 800 - 804. [Abstract] [Full Text]