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Microbiology 146 (2000), 823-828
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Microbiology (2000), 146, 823-828.
© 2000 Society for General Microbiology

Genomics

RT-PCR as a tool for systematic transcriptional analysis of large regions of the Bacillus subtilis genome

Alicia Hernández1, Angélica Figueroa1, Luis A. Rivas1, Víctor Parro1 and Rafael P. Mellado1

Centro Nacional de Biotecnología (CSIC), Campus de Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain1

Author for correspondence: Rafael P. Mellado. Tel: +34 91 5854547. Fax: +34 91 5854506. e-mail: rpmellado{at}cnb.uam.es

Transcriptional analysis of five different regions of the Bacillus subtilis 168 genome, comprising a total of 175 kb encoding newly identified genes, was carried out using the RT-PCR technique as part of the functional analysis of the whole genome of this bacterium. Amplification of mRNA fragments allowed the detection of both highly and poorly transcribed genes covering 81% of putative ORFs, and also the monitoring of variations in the expression level among genes differentially expressed during particular bacterial growth phases.

Keywords: Bacillus subtilis, transcription, new genes




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