| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Genetics and Molecular Biology |
Department of Genetics1 and Department of Microbiology and Immunology2, University of Leicester, Leicester LE1 7RH, UK
Author for correspondence: Annette M. Cashmore. Tel: +44 116 252 3439. Fax: +44 116 252 5101. e-mail: amc19{at}le.ac.uk
Candida albicans, like other pathogens, has to compete with the host for a limited supply of available iron. Consequently, iron acquisition is likely to be an important factor for the growth, survival and virulence of this organism. It was previously demonstrated that C. albicans has a surface-associated ferric reductase similar to that of Saccharomyces cerevisiae. Therefore, functional rescue of a S. cerevisiae fre1 mutant was used to isolate a C. albicans ferric reductase gene (CFL1). This gene has been previously identified. However, the workers had not observed any functional reductase activity associated with the gene. The discrepancy with the findings in this report appears to be due to the clone previously reported carrying a non-contiguous piece of C. albicans DNA. Results shown in this paper demonstrate that CFL1 transcription is regulated in response to levels of iron and copper. This is the first demonstration of a functional ferric reductase gene from C. albicans.
Keywords: Candida albicans, CFL1, iron uptake, ferric reductase, Saccharomyces cerevisiae
Abbreviations: BCS, bathocuproine disulphonic acid; BPS, bathophenanthroline disulphonic acid; FNR, ferredoxin-NADP+ reductase
The EMBL accession number for the sequence reported in this paper is AJ387722.
This article has been cited by other articles:
|
|
 |
|
  E. M. Rees and D. J. Thiele Identification of a Vacuole-associated Metalloreductase and Its Role in Ctr2-mediated Intracellular Copper Mobilization J. Biol. Chem., July 27, 2007; 282(30): 21629 - 21638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Prasad, A. Chandra, C. K. Mukhopadhyay, and R. Prasad Unexpected Link between Iron and Drug Resistance of Candida spp.: Iron Depletion Enhances Membrane Fluidity and Drug Diffusion, Leading to Drug-Susceptible Cells Antimicrob. Agents Chemother., November 1, 2006; 50(11): 3597 - 3606. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Gebhart, A. K. Bahrami, and A. Sil Identification of a Copper-Inducible Promoter for Use in Ectopic Expression in the Fungal Pathogen Histoplasma capsulatum Eukaryot. Cell, June 1, 2006; 5(6): 935 - 944. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. B. Knight, G. Vilaire, E. Lesuisse, and A. Dancis Iron Acquisition from Transferrin by Candida albicans Depends on the Reductive Pathway Infect. Immun., September 1, 2005; 73(9): 5482 - 5492. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Marvin, R. P. Mason, and A. M. Cashmore The CaCTR1 gene is required for high-affinity iron uptake and is transcriptionally controlled by a copper-sensing transactivator encoded by CaMAC1 Microbiology, July 1, 2004; 150(7): 2197 - 2208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Niewerth, D. Kunze, M. Seibold, M. Schaller, H. C. Korting, and B. Hube Ciclopirox Olamine Treatment Affects the Expression Pattern of Candida albicans Genes Encoding Virulence Factors, Iron Metabolism Proteins, and Drug Resistance Factors Antimicrob. Agents Chemother., June 1, 2003; 47(6): 1805 - 1817. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Marvin, P. H. Williams, and A. M. Cashmore The Candida albicansCTR1 gene encodes a functional copper transporter Microbiology, June 1, 2003; 149(6): 1461 - 1474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Santos, N. Buisson, S. Knight, A. Dancis, J.-M. Camadro, and E. Lesuisse Haemin uptake and use as an iron source by Candida albicans: role of CaHMX1-encoded haem oxygenase Microbiology, March 1, 2003; 149(3): 579 - 588. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. La Fontaine, J. M. Quinn, S. S. Nakamoto, M. D. Page, V. Gohre, J. L. Moseley, J. Kropat, and S. Merchant Copper-Dependent Iron Assimilation Pathway in the Model Photosynthetic Eukaryote Chlamydomonas reinhardtii Eukaryot. Cell, October 1, 2002; 1(5): 736 - 757. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-J. Hu, C. Bai, X.-D. Zheng, Y.-M. Wang, and Y. Wang Characterization and Functional Analysis of the Siderophore-Iron Transporter CaArn1p in Candida albicans J. Biol. Chem., August 16, 2002; 277(34): 30598 - 30605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. B. Knight, E. Lesuisse, R. Stearman, R. D. Klausner, and A. Dancis Reductive iron uptake by Candida albicans: role of copper, iron and the TUP1 regulator Microbiology, January 1, 2002; 148(1): 29 - 40. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
