HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bey, S.-J. Articles by Chen, C. W. Search for Related Content PubMed PubMed Citation Articles by Bey, S.-J. Articles by Chen, C. W. Agricola Articles by Bey, S.-J. Articles by Chen, C. W.

The homologous terminal sequence of the Streptomyces lividans chromosome and SLP2 plasmid

Institute of Genetics, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan¹
Department of Chemistry, Chung-Yuan Christian University, Chung-Li, Taiwan²

Author for correspondence: Carton W. Chen. Tel: +886 2 2826 7040. Fax: +886 2 2826 4930. e-mail: cwchen{at}ym.edu.tw

The chromosome of Streptomyces lividans shares 15·4 kb homology with one end of the linear plasmid SLP2, consisting of a 10·1 kb terminal sequence followed by the 5·3 kb transposable element Tn4811. The 10·1 kb terminal sequence was determined. The mean G+C content of this sequence is 67·9 mol% with a striking G vs C bias in the last kb. The terminal 232 nt contained 10 palindromic sequences with potential to form complex secondary structures. One typical Streptomyces coding sequence (designated ORF1) of 2643 bp was predicted in the determined sequence. The amino acid sequence of the ORF1 product contained a DEAH helicase motif, and exhibited similarity to type I restriction enzyme HsdR subunits in the database, suggesting a possible role in replication of the telomeres. However, all the ORF1 sequences on the chromosome and SLP2 could be simultaneously knocked out by targeted recombination without affecting the viability of the cells and the linearity of the chromosome and SLP2. This ruled out ORF1 as an essential component in the maintenance of the linear chromosome and plasmids.

Keywords: Streptomyces, chromosome, linear plasmid, telomere, helicase

Abbreviations: TIR, terminal inverted repeat; TP, terminal protein

The GenBank accession number for the sequence determined in this work is AF194023.

^a Present address: Cell and Developmental Biology, Graduate Group Complex, University of California, Davis, CA 95616, USA.

This article has been cited by other articles:

				F. Choulet, A. Gallois, B. Aigle, S. Mangenot, C. Gerbaud, C. Truong, F.-X. Francou, F. Borges, C. Fourrier, M. Guerineau, et al. Intraspecific Variability of the Terminal Inverted Repeats of the Linear Chromosome of Streptomyces ambofaciens. J. Bacteriol., September 1, 2006; 188(18): 6599 - 6610. [Abstract] [Full Text] [PDF]

				C. Konig, D. Eulberg, J. Groning, S. Lakner, V. Seibert, S. R. Kaschabek, and M. Schlomann A linear megaplasmid, p1CP, carrying the genes for chlorocatechol catabolism of Rhodococcus opacus 1CP Microbiology, September 1, 2004; 150(9): 3075 - 3087. [Abstract] [Full Text] [PDF]

				E. Grohmann, G. Muth, and M. Espinosa Conjugative Plasmid Transfer in Gram-Positive Bacteria Microbiol. Mol. Biol. Rev., June 1, 2003; 67(2): 277 - 301. [Abstract] [Full Text] [PDF]