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Pathogenicity and Medical Microbiology |
Microbial Collection and Screening Laboratory, Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 1176091
Author for correspondence: Yue Wang. Tel: +65 778 3207. Fax: +65 7791117. e-mail: mcbwangy{at}imcb.nus.edu.sg
The isolation and functional characterization of a Candida albicans Na+/H+ antiporter gene, CNH1, is reported here. The gene encodes a protein of 840 amino acids that exhibits high levels of similarity in sequence, size, and structural and functional domains to a group of known Na+/H+ antiporters of fungi. The CNH1 gene is able to functionally complement the salt-sensitivity of a Saccharomyces cerevisiae ena1 nha1 mutant, and mutations of two conserved aspartate residues to asparagines in the putative Na+-binding site abolish this activity. Deletion of CNH1 results in retardation of growth and a highly elongated morphology in a significant fraction of cells under conditions that normally support yeast growth. These results indicate that CNH1 has a role in Na+ and H+ transport, salt-tolerance, and morphogenesis.
Keywords: Na+/H+ antiporter, Candida albicans, salt tolerance, morphogenesis, gene disruption
Abbreviations: GFP, green fluorescent protein
The GenBank accession number for the sequence reported in this work is AF128238.
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