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Microbiology (2000), 146, 1163-1169.
© 2000 Society for General Microbiology

Genetics and Molecular Biology

Transposition in Lactobacillus sakei: inactivation of a second lactocin S operon by the insertion of IS1520, a new member of the IS3 family of insertion sequences

Morten Skaugen1 and Ingolf F. Nes1

Laboratory of Microbial Gene Technology, Agricultural University of Norway, PO Box 5051, N-1432 s-NLH, Norway1

Author for correspondence: Morten Skaugen. Tel: +47 64949466. Fax: +47 64941465. e-mail: morten.skaugen{at}ikb.nlh.no

The analysis of spontaneous bacteriocin-negative mutants has led to the identification and characterization of a new, transpositionally active, insertion sequence of the IS3 family in the lactocin-S-producing Lactobacillus sakei strain L45. The element, which has been designated IS1520, is 1302 bp long with 10 bp perfect inverted repeat ends and generates direct repeats of a trinucleotide of target sequence upon transposition to the lactocin S locus. IS1520 encodes two consecutive, partially overlapping, major ORFs, which are frameshifted in a manner typical of the IS3 family. Despite a low overall DNA sequence similarity, the putative frameshifting region of IS1520 is highly similar to the corresponding region of IS1163, which is a related element previously shown to be active in L. sakei L45.

Keywords: transposition, insertion sequence, lantibiotic

The EMBL accession number for the IS1520 nucleotide sequence is AJ250958.




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