| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Development and Structure |
Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka 558-8585, Japan1
Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria2
Author for correspondence: Makoto Miyata. Tel: +81 6 6605 3157. Fax: +81 6 6605 3158. e-mail: miyata{at}sci.osaka-cu.ac.jp
The present study characterizes gliding motility mutants of Mycoplasma mobile which were obtained by UV irradiation. They were identified by their abnormal colony shapes in 0·1% agar medium, showing a reduced number of satellite colonies compared to the wild-type. A total of ten mutants were isolated based on their colony phenotype. Using dark-field and electron microscopy, two classes of mutants, group I and group II, were defined. Cells of group I mutants had irregular, flexible and sometimes elongated head-like structures and showed a tendency to aggregate. Neither binding to glass nor gliding motility was observed in these mutants. Cells of group II mutants were rather spherical in shape, with the long axis reduced to 80% and the short axis enlarged to 120% of that of wild-type cells, respectively. Their gliding speed was 20% faster than that of wild-type cells. Three of the ten mutants remained unclassified. Mutant m6 had a reduced binding activity to glass and a reduced gliding motility with 50% of the speed of the wild-type strain. The ability of wild-type and mutant colonies to adsorb erythrocytes was found to correlate with the binding activity required for gliding, indicating that mycoplasma gliding depends on cytadherence-associated components. Finally, the ability to form microcolonies on surfaces was shown to correlate with the gliding activity, suggesting a certain role of gliding motility in the parasitic life-cycle of mycoplasmas.
Keywords: mollicutes, Mycoplasma mobile, gliding mutants, cell morphology, cell adhesion
a Present address: Department of Bacteriology, Kurume University, School of Medicine, Asahimachi 67, Kurume 830-0011, Japan.
This article has been cited by other articles:
|
|
 |
|
  D. Nakane and M. Miyata Cytoskeletal "jellyfish" structure of Mycoplasma mobile PNAS, December 4, 2007; 104(49): 19518 - 19523. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Nagai and M. Miyata Gliding Motility of Mycoplasma mobile Can Occur by Repeated Binding to N-Acetylneuraminyllactose (Sialyllactose) Fixed on Solid Surfaces. J. Bacteriol., September 1, 2006; 188(18): 6469 - 6475. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Adan-Kubo, A. Uenoyama, T. Arata, and M. Miyata Morphology of Isolated Gli349, a Leg Protein Responsible for Mycoplasma mobile Gliding via Glass Binding, Revealed by Rotary Shadowing Electron Microscopy. J. Bacteriol., April 1, 2006; 188(8): 2821 - 2828. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. F. Braun, M. K. Khubbar, D. A. Saffarini, and M. J. McBride Flavobacterium johnsoniae Gliding Motility Genes Identified by mariner Mutagenesis J. Bacteriol., October 15, 2005; 187(20): 6943 - 6952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. W. Charon Mycoplasma takes a walk PNAS, September 27, 2005; 102(39): 13713 - 13714. [Full Text] [PDF]
|
|
|
|
|
|
B. M. Hasselbring, J. L. Jordan, and D. C. Krause Mutant Analysis Reveals a Specific Requirement for Protein P30 in Mycoplasma pneumoniae Gliding Motility J. Bacteriol., September 15, 2005; 187(18): 6281 - 6289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Uenoyama and M. Miyata From the Cover: Gliding ghosts of Mycoplasma mobile PNAS, September 6, 2005; 102(36): 12754 - 12758. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Uenoyama and M. Miyata Identification of a 123-Kilodalton Protein (Gli123) Involved in Machinery for Gliding Motility of Mycoplasma mobile J. Bacteriol., August 15, 2005; 187(16): 5578 - 5584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Seto, A. Uenoyama, and M. Miyata Identification of a 521-Kilodalton Protein (Gli521) Involved in Force Generation or Force Transmission for Mycoplasma mobile Gliding J. Bacteriol., May 15, 2005; 187(10): 3502 - 3510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. F. Braun and M. J. McBride Flavobacterium johnsoniae GldJ Is a Lipoprotein That Is Required for Gliding Motility J. Bacteriol., April 15, 2005; 187(8): 2628 - 2637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Seto, T. Kenri, T. Tomiyama, and M. Miyata Involvement of P1 Adhesin in Gliding Motility of Mycoplasma pneumoniae as Revealed by the Inhibitory Effects of Antibody under Optimized Gliding Conditions J. Bacteriol., March 1, 2005; 187(5): 1875 - 1877. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kusumoto, S. Seto, J. D. Jaffe, and M. Miyata Cell surface differentiation of Mycoplasma mobile visualized by surface protein localization Microbiology, December 1, 2004; 150(12): 4001 - 4008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kenri, S. Seto, A. Horino, Y. Sasaki, T. Sasaki, and M. Miyata Use of Fluorescent-Protein Tagging To Determine the Subcellular Localization of Mycoplasma pneumoniae Proteins Encoded by the Cytadherence Regulatory Locus J. Bacteriol., October 15, 2004; 186(20): 6944 - 6955. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Jaffe, N. Stange-Thomann, C. Smith, D. DeCaprio, S. Fisher, J. Butler, S. Calvo, T. Elkins, M. G. FitzGerald, N. Hafez, et al. The Complete Genome and Proteome of Mycoplasma mobile Genome Res., August 1, 2004; 14(8): 1447 - 1461. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Jaffe, M. Miyata, and H. C. Berg Energetics of Gliding Motility in Mycoplasma mobile J. Bacteriol., July 1, 2004; 186(13): 4254 - 4261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Miyata and J. D. Petersen Spike Structure at the Interface between Gliding Mycoplasma mobile Cells and Glass Surfaces Visualized by Rapid-Freeze-and-Fracture Electron Microscopy J. Bacteriol., July 1, 2004; 186(13): 4382 - 4386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Uenoyama, A. Kusumoto, and M. Miyata Identification of a 349-Kilodalton Protein (Gli349) Responsible for Cytadherence and Glass Binding during Gliding of Mycoplasma mobile J. Bacteriol., March 1, 2004; 186(5): 1537 - 1545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Seto and M. Miyata Attachment Organelle Formation Represented by Localization of Cytadherence Proteins and Formation of the Electron-Dense Core in Wild-Type and Mutant Strains of Mycoplasma pneumoniae J. Bacteriol., February 1, 2003; 185(3): 1082 - 1091. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Miyata, W. S. Ryu, and H. C. Berg Force and Velocity of Mycoplasma mobile Gliding J. Bacteriol., April 1, 2002; 184(7): 1827 - 1831. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. B. Bourret, N. W. Charon, A. M. Stock, and A. H. West Bright Lights, Abundant Operons--Fluorescence and Genomic Technologies Advance Studies of Bacterial Locomotion and Signal Transduction: Review of the BLAST Meeting, Cuernavaca, Mexico, 14 to 19 January 2001 J. Bacteriol., January 1, 2002; 184(1): 1 - 17. [Full Text] [PDF]
|
|
|
|
|
|
S. Seto, G. Layh-Schmitt, T. Kenri, and M. Miyata Visualization of the Attachment Organelle and Cytadherence Proteins of Mycoplasma pneumoniae by Immunofluorescence Microscopy J. Bacteriol., March 1, 2001; 183(5): 1621 - 1630. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
