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Genetics and Molecular Biology |
Unité des Agents Antibactériens1 and Laboratoire de Génomique des Micro-organismes Pathogènes2, Institut Pasteur, 75724 Paris Cedex 15, France
Author for correspondence: Marc Galimand. Tel: +33 1 45 68 83 18. Fax: +33 1 45 68 83 19. e-mail: galimand{at}pasteur.fr
Transfer of VanB-type resistance to glycopeptides among enterococci has been reported to be associated with the movement of large chromosomal genetic elements or of plasmids. The authors report the characterization of the 34 kb transposon Tn1549 borne by a plasmid related to pAD1 and conferring vancomycin resistance in clinical isolates of Enterococcus spp. Tn1549 contained 30 ORFs and appeared to be organized like the Tn916 family of conjugative transposons into three functional regions: (i) the right end, implicated in the excision–integration process; (ii) the central part, in which the vanB2 operon replaces the tet(M) gene; and (iii) the left extremity, in which eight of the 18 ORFs could be implicated in the conjugative transfer.
Keywords: Tn1549, glycopeptide resistance, VanB phenotype
The GenBank accession number for the 33803 bp sequence of Tn1549 is AJ192329.
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