| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Genetics and Molecular Biology |
Unité des Agents Antibactériens1 and Laboratoire de Génomique des Micro-organismes Pathogènes2, Institut Pasteur, 75724 Paris Cedex 15, France
Author for correspondence: Marc Galimand. Tel: +33 1 45 68 83 18. Fax: +33 1 45 68 83 19. e-mail: galimand{at}pasteur.fr
Transfer of VanB-type resistance to glycopeptides among enterococci has been reported to be associated with the movement of large chromosomal genetic elements or of plasmids. The authors report the characterization of the 34 kb transposon Tn1549 borne by a plasmid related to pAD1 and conferring vancomycin resistance in clinical isolates of Enterococcus spp. Tn1549 contained 30 ORFs and appeared to be organized like the Tn916 family of conjugative transposons into three functional regions: (i) the right end, implicated in the excision–integration process; (ii) the central part, in which the vanB2 operon replaces the tet(M) gene; and (iii) the left extremity, in which eight of the 18 ORFs could be implicated in the conjugative transfer.
Keywords: Tn1549, glycopeptide resistance, VanB phenotype
The GenBank accession number for the 33803 bp sequence of Tn1549 is AJ192329.
This article has been cited by other articles:
|
|
 |
|
  A. San Millan, F. Depardieu, S. Godreuil, and P. Courvalin VanB-Type Enterococcus faecium Clinical Isolate Successively Inducibly Resistant to, Dependent on, and Constitutively Resistant to Vancomycin Antimicrob. Agents Chemother., May 1, 2009; 53(5): 1974 - 1982. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Zheng, H. Tomita, T. Inoue, and Y. Ike Isolation of VanB-Type Enterococcus faecalis Strains from Nosocomial Infections: First Report of the Isolation and Identification of the Pheromone-Responsive Plasmids pMG2200, Encoding VanB-Type Vancomycin Resistance and a Bac41-Type Bacteriocin, and pMG2201, Encoding Erythromycin Resistance and Cytolysin (Hly/Bac) Antimicrob. Agents Chemother., February 1, 2009; 53(2): 735 - 747. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Kazimierczak, M. T. Rincon, A. J. Patterson, J. C. Martin, P. Young, H. J. Flint, and K. P. Scott A New Tetracycline Efflux Gene, tet(40), Is Located in Tandem with tet(O/32/O) in a Human Gut Firmicute Bacterium and in Metagenomic Library Clones Antimicrob. Agents Chemother., November 1, 2008; 52(11): 4001 - 4009. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Saha, A. K. Singh, A. Ghosh, and M. Bal Identification and characterization of a vancomycin-resistant Staphylococcus aureus isolated from Kolkata (South Asia) J. Med. Microbiol., January 1, 2008; 57(1): 72 - 79. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-C. Domingo, A. Huletsky, R. Giroux, F. J. Picard, and M. G. Bergeron vanD and vanG-Like Gene Clusters in a Ruminococcus Species Isolated from Human Bowel Flora Antimicrob. Agents Chemother., November 1, 2007; 51(11): 4111 - 4117. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kurokawa, T. Itoh, T. Kuwahara, K. Oshima, H. Toh, A. Toyoda, H. Takami, H. Morita, V. K. Sharma, T. P. Srivastava, et al. Comparative Metagenomics Revealed Commonly Enriched Gene Sets in Human Gut Microbiomes DNA Res, October 16, 2007; (2007) dsm018v2. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Beltrametti, A. Consolandi, L. Carrano, F. Bagatin, R. Rossi, L. Leoni, E. Zennaro, E. Selva, and F. Marinelli Resistance to Glycopeptide Antibiotics in the Teicoplanin Producer Is Mediated by van Gene Homologue Expression Directing the Synthesis of a Modified Cell Wall Peptidoglycan Antimicrob. Agents Chemother., April 1, 2007; 51(4): 1135 - 1141. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Torres, S. Escobar, A. Portillo, L. Torres, A. Rezusta, F. Ruiz-Larrea, M. J. Revillo, C. Aspiroz, and M. Zarazaga Detection of clonally related vanB2-containing Enterococcus faecium strains in two Spanish hospitals. J. Med. Microbiol., September 1, 2006; 55(Pt 9): 1237 - 1243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Kazimierczak, H. J. Flint, and K. P. Scott Comparative Analysis of Sequences Flanking tet(W) Resistance Genes in Multiple Species of Gut Bacteria. Antimicrob. Agents Chemother., August 1, 2006; 50(8): 2632 - 2639. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Launay, S. A. Ballard, P. D. R. Johnson, M. L. Grayson, and T. Lambert Transfer of Vancomycin Resistance Transposon Tn1549 from Clostridium symbiosum to Enterococcus spp. in the Gut of Gnotobiotic Mice Antimicrob. Agents Chemother., March 1, 2006; 50(3): 1054 - 1062. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. B. Rice, L. L. Carias, S. Rudin, V. Lakticova, A. Wood, and R. Hutton-Thomas Enterococcus faecium Low-Affinity pbp5 Is a Transferable Determinant Antimicrob. Agents Chemother., December 1, 2005; 49(12): 5007 - 5012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Guardabassi, B. Perichon, J. van Heijenoort, D. Blanot, and P. Courvalin Glycopeptide Resistance vanA Operons in Paenibacillus Strains Isolated from Soil Antimicrob. Agents Chemother., October 1, 2005; 49(10): 4227 - 4233. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. B. Rice, L. L. Carias, S. Marshall, S. D. Rudin, and R. Hutton-Thomas Tn5386, a Novel Tn916-Like Mobile Element in Enterococcus faecium D344R That Interacts with Tn916 To Yield a Large Genomic Deletion J. Bacteriol., October 1, 2005; 187(19): 6668 - 6677. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-J. Lu, T.-Y. Chang, C.-L. Perng, and S.-Y. Lee The vanB2 Gene Cluster of the Majority of Vancomycin-Resistant Enterococcus faecium Isolates from Taiwan Is Associated with the pbp5 Gene and Is Carried by Tn5382 Containing a Novel Insertion Sequence Antimicrob. Agents Chemother., September 1, 2005; 49(9): 3937 - 3939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Ballard, K. K. Pertile, M. Lim, P. D. R. Johnson, and M. L. Grayson Molecular Characterization of vanB Elements in Naturally Occurring Gut Anaerobes Antimicrob. Agents Chemother., May 1, 2005; 49(5): 1688 - 1694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-C. Domingo, A. Huletsky, A. Bernal, R. Giroux, D. K. Boudreau, F. J. Picard, and M. G. Bergeron Characterization of a Tn5382-like transposon containing the vanB2 gene cluster in a Clostridium strain isolated from human faeces J. Antimicrob. Chemother., April 1, 2005; 55(4): 466 - 474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Ballard, E. A. Grabsch, P. D. R. Johnson, and M. L. Grayson Comparison of Three PCR Primer Sets for Identification of vanB Gene Carriage in Feces and Correlation with Carriage of Vancomycin-Resistant Enterococci: Interference by vanB-Containing Anaerobic Bacilli Antimicrob. Agents Chemother., January 1, 2005; 49(1): 77 - 81. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Melville, R. Brunel, H. J. Flint, and K. P. Scott The Butyrivibrio fibrisolvens tet(W) Gene Is Carried on the Novel Conjugative Transposon TnB1230, Which Contains Duplicated Nitroreductase Coding Sequences J. Bacteriol., June 1, 2004; 186(11): 3656 - 3659. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Pavlovic, V. Burrus, B. Gintz, B. Decaris, and G. Guedon Evolution of genomic islands by deletion and tandem accretion by site-specific recombination: ICESt1-related elements from Streptococcus thermophilus Microbiology, April 1, 2004; 150(4): 759 - 774. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. H. Dahl and A. Sundsfjord Transferable vanB2 Tn5382-Containing Elements in Fecal Streptococcal Strains from Veal Calves Antimicrob. Agents Chemother., August 1, 2003; 47(8): 2579 - 2583. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Grohmann, G. Muth, and M. Espinosa Conjugative Plasmid Transfer in Gram-Positive Bacteria Microbiol. Mol. Biol. Rev., June 1, 2003; 67(2): 277 - 301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. H. Dahl, T. P. Rokenes, E. W. Lundblad, and A. Sundsfjord Nonconjugative Transposition of the vanB-Containing Tn5382-Like Element in Enterococcus faecium Antimicrob. Agents Chemother., February 1, 2003; 47(2): 786 - 789. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Tomita, C. Pierson, S. K. Lim, D. B. Clewell, and Y. Ike Possible Connection between a Widely Disseminated Conjugative Gentamicin Resistance (pMG1-Like) Plasmid and the Emergence of Vancomycin Resistance in Enterococcus faecium J. Clin. Microbiol., September 1, 2002; 40(9): 3326 - 3333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Umeda, F. Garnier, P. Courvalin, and M. Galimand Association between the vanB2 glycopeptide resistance operon and Tn1549 in enterococci from France J. Antimicrob. Chemother., August 1, 2002; 50(2): 253 - 256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Whittle, N. B. Shoemaker, and A. A. Salyers Characterization of Genes Involved in Modulation of Conjugal Transfer of the Bacteroides Conjugative Transposon CTnDOT J. Bacteriol., July 15, 2002; 184(14): 3839 - 3847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. DEMERTZI, M-F.I. PALEPOU, M.E. KAUFMANN, A. AVLAMIS, and N. WOODFORD Characterisation of VanA and VanB elements from glycopeptide-resistant Enterococcus faecium from Greece J. Med. Microbiol., August 1, 2001; 50(8): 682 - 687. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Showsh, E. H. De Boever, and D. B. Clewell Vancomycin Resistance Plasmid in Enterococcus faecalis That Encodes Sensitivity to a Sex Pheromone Also Produced by Staphylococcus aureus Antimicrob. Agents Chemother., July 1, 2001; 45(7): 2177 - 2178. [Full Text] [PDF]
|
|
|
|
|
|
B. Casadewall, P. E. Reynolds, and P. Courvalin Regulation of Expression of the vanD Glycopeptide Resistance Gene Cluster from Enterococcus faecium BM4339 J. Bacteriol., June 1, 2001; 183(11): 3436 - 3446. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. A. Lewis and G. F. Hatfull Control of directionality in integrase-mediated recombination: examination of recombination directionality factors (RDFs) including Xis and Cox proteins Nucleic Acids Res., June 1, 2001; 29(11): 2205 - 2216. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Østergaard, L. Brøndsted, and F. K. Vogensen Identification of a Replication Protein and Repeats Essential for DNA Replication of the Temperate Lactococcal Bacteriophage TP901-1 Appl. Envir. Microbiol., February 1, 2001; 67(2): 774 - 781. [Abstract] [Full Text]
|
|
|
|
|
|
K. F. McGregor, C. Nolan, H.-K. Young, M.-F. I. Palepou, L. Tysall, and N. Woodford Prevalence of the vanB2 Gene Cluster in VanB Glycopeptide-Resistant Enterococci in the United Kingdom and the Republic of Ireland and Its Association with a Tn5382-Like Element Antimicrob. Agents Chemother., January 1, 2001; 45(1): 367 - 368. [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
