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Microbiology 146 (2000), 1555-1563
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Microbiology (2000), 146, 1555-1563.
© 2000 Society for General Microbiology

Genetics and Molecular Biology

A novel ß-glucoside-specific PTS locus from Streptococcus mutans that is not inhibited by glucose

Christopher K. Cote1, Dennis Cvitkovitcha,2, Arnold S. Bleiweis2 and Allen L. Honeyman1

Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, 12901 Bruce B. Downs Boulevard, Tampa, FL 33612, USA1
Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL 32611, USA2

Author for correspondence: Allen L. Honeyman. Tel: +1 813 974 2362. Fax: +1 813 974 4151. e-mail: ahoneyma{at}com1.med.usf.edu

A regulon from Streptococcus mutans that plays a role in the utilization of ß-glucosides has been isolated, sequenced and subjected to sequence analysis. This regulon encodes a ß-glucoside-specific Enzyme II (EII) component (bglP) of the phosphoenolpyruvate-dependent phosphotransferase system (PTS) and a phospho-ß-glucosidase (bglA) which is responsible for the breakdown of the phospho-ß-glucosides within the cell. Both the bglP and bglA gene products have significant similarity with proteins that have similar functions from Clostridium longisporum, Listeria monocytogenes, Erwinia chrysanthemi, Escherichia coli, Klebsellia oxytoca and Bacillus subtilis. The potential functions of the BglP and BglA proteins are supported by phenotypic data from both S. mutans and E. coli. A chromosomal deletion in S. mutans spanning the bglP and bglA genes resulted in a strain that was unable to hydrolyse the ß-glucoside aesculin in the presence of glucose. When glucose was removed from the medium, the deletion strain regained the ability to break down aesculin. These data suggest that S. mutans possesses an alternative mechanism from the one described in this report for breaking down ß-glucosides. This second mechanism was repressed by glucose while the regulon described here was not. Complementation studies in E. coli CC118 also suggest a potential role for this regulon in the utilization of other ß-glucosides. When a plasmid containing the 8 kb ß-glucoside-specific regulon was transformed into E. coli CC118, the transformed strain was able to break down the ß-glucoside arbutin.

Keywords: Streptococcus mutans, ß-glucosides, aesculin, phosphoenolpyruvate-dependent phosphotransferase system

Abbreviations: CTAB, cetyltrimethylammonium bromide; EII, Enzyme II; PTS, phosphoenolpyruvate-dependent phosphotransferase system

The GenBank accession number for the sequence reported in this paper is AF206272.

a Present address: Dental Research Institute, University of Toronto School of Dentistry, Toronto, Ontario, Canada.




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