HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Tomlin, G. C. Articles by Oliver, S. G. Search for Related Content PubMed PubMed Citation Articles by Tomlin, G. C. Articles by Oliver, S. G. Agricola Articles by Tomlin, G. C. Articles by Oliver, S. G.

Suppression of sorbitol dependence in a strain bearing a mutation in the SRB1/PSA1/VIG9 gene encoding GDP-mannose pyrophosphorylase by PDE2 overexpression suggests a role for the Ras/cAMP signal-transduction pathway in the control of yeast cell-wall biogenesis

School of Biological Sciences, 2.205 Stopford Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK¹
Department of Biomolecular Sciences, UMIST, PO Box 88, Manchester M60 1QD, UK²

Author for correspondence: Stephen G. Oliver. Tel: +44 161 606 7260. Fax: +44 161 606 7360. e-mail: steve.oliver{at}man.ac.uk

Complementation studies and allele replacement in Saccharomyces cerevisiae revealed that PSA1/VIG9, an essential gene that encodes GDP-mannose pyrophosphorylase, is the wild-type SRB1 gene. Cloning and sequencing of the srb1-1 allele showed that it determines a single amino acid change from glycine to aspartic acid at residue 276 (srb1^D276). Genetic evidence is presented showing that at least one further mutation is required for the sorbitol dependence of srb1^D276. A previously reported complementing gene, which this study has now identified as PDE2, is a multi-copy suppressor of sorbitol dependence and is not, as was previously suggested, the SRB1 gene. srb and pde2 mutants share a number of phenotypes, including lysis upon hypotonic shock and enhanced transformability. These data are consistent with the idea that the Ras/cAMP pathway might modulate cell-wall construction.

Keywords: SRB1/PSA1/VIG9/YDL055c, PDE2, cell-wall integrity, GDP-mannose

Abbreviations: GPI, glycosyl-phosphatidyl-inositol; MAP, mitogen-activated protein; PKA, cAMP-dependent protein kinase; STRE, stress-response element

^a Present address: Millipore, BioProcess Division, Consett, Durham DH8 6SZ, UK.

This article has been cited by other articles:

				S. Biswas, P. Van Dijck, and A. Datta Environmental Sensing and Signal Transduction Pathways Regulating Morphopathogenic Determinants of Candida albicans Microbiol. Mol. Biol. Rev., June 1, 2007; 71(2): 348 - 376. [Abstract] [Full Text] [PDF]

				L. Walsh, P.W. Hastwell, P.O. Keenan, A.W. Knight, N. Billinton, and R.M. Walmsley Genetic modification and variations in solvent increase the sensitivity of the yeast RAD54-GFP genotoxicity assay Mutagenesis, September 1, 2005; 20(5): 317 - 327. [Abstract] [Full Text] [PDF]

				H. Imazu and H. Sakurai Saccharomyces cerevisiae Heat Shock Transcription Factor Regulates Cell Wall Remodeling in Response to Heat Shock Eukaryot. Cell, June 1, 2005; 4(6): 1050 - 1056. [Abstract] [Full Text] [PDF]

				D. Harcus, A. Nantel, A. Marcil, T. Rigby, and M. Whiteway Transcription Profiling of Cyclic AMP Signaling in Candida albicans Mol. Biol. Cell, October 1, 2004; 15(10): 4490 - 4499. [Abstract] [Full Text] [PDF]

				D. L. Jones, J. Petty, D. C. Hoyle, A. Hayes, E. Ragni, L. Popolo, S. G. Oliver, and L. I. Stateva Transcriptome profiling of a Saccharomyces cerevisiae mutant with a constitutively activated Ras/cAMP pathway Physiol Genomics, December 16, 2003; 16(1): 107 - 118. [Abstract] [Full Text] [PDF]

				W. H. Jung and L. I. Stateva The cAMP phosphodiesterase encoded by CaPDE2 is required for hyphal development in Candida albicans Microbiology, October 1, 2003; 149(10): 2961 - 2976. [Abstract] [Full Text] [PDF]