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Microbiology 146 (2000), 2317-2323
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Microbiology (2000), 146, 2317-2323.
© 2000 Society for General Microbiology

Biochemistry

Binding of glycoglycerolipid derived from membranes of Acholeplasma laidlawii PG8 and synthetic analogues to lymphoid cells

Saori Toujima1, Koichi Kuwano1, Ye Zhang1, Naoyuki Fujimoto1, Masahiro Hirama2, Tohru Oishi2, Sumiko Fukuda2, Yoko Nagumo2, Hiroto Imai2, Tsukasa Kikuchi2 and Sumio Arai1

Department of Bacteriology, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan1
Department of Chemistry, Graduate School of Science, Tohoku University Aoba, Aramaki aza, Aoba-ku, Sendai 980-8578, Japan2

Author for correspondence: Sumio Arai. Tel: +81 942 31 7548. Fax: +81 942 31 0343. e-mail: sumiyuku{at}mx2.tiki.ne.jp

A component that binds to human lymphoid cells was isolated from the membranes of Acholeplasma laidlawii PG8. The component was extracted using the Bligh–Dyer method and purified using a silica-gel column and TLC. The active component was identified as 3-O-[2'-O-({alpha}-D-glucopyranosyl)- 6'-O-acyl-{alpha}-D-glucopyranosyl]-1,2-di-O- acyl-sn-glycerol (GAGDG) using 1H- and 13C-NMR and GC-MS. The compositions of the major saturated fatty acids were nC 14 (17·8%), isoC14 (10·7%) and nC 16 (34·9%) as determined by GC-MS. The amounts of unsaturated species were less than 10% of those of the corresponding saturated acids. GAGDGs which have three tetradecanoyl groups were synthesized. These synthetic GAGDGs, as well as GAGDGs derived from A. laidlawii membranes, had a high binding affinity for MOLT-4 and HUT-78 (human T cell lines), Raji (a B cell line), HL-60 (a monoblastoid cell line) and primary cultured human T cells. The binding affinities of GAGDGs with an isoC14 acyl group was higher than those with nC14 and nC16 acyl groups. The binding to lymphoid cells reveals a novel biological activity of GAGDGs.

Keywords: Acholeplasma laidlawii, adherence, glycoglycerolipids, lymphoid cells

Abbreviations: GAGDG, 3-O-[2'-O-({alpha}-D-glucopyranosyl)- 6'-O-acyl-{alpha}-D-glucopyranosyl]-1,2-di-O- acyl-sn-glycerol






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