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Genetics and Molecular Biology |
1yna Konopa2
grzyn2,3
Laboratory of Molecular Biology (affiliated with the University of Gda
sk), Institute of Biochemistry and Biophysics, Polish Academy of Sciences, K
adki 24, 80-822 Gdask, Poland1
Department of Molecular Biology, University of Gdask, Kladki 24, 80-822 Gdask, Poland2
Marine Biology Center, Polish Academy of Sciences, 
w. Wojciecha 5, 81-347 Gdynia, Poland3
Author for correspondence: Grzegorz Wgrzyn. Tel: +48 58 346 3014. Fax: +48 58 301 0072. e-mail: wegrzyn{at}biotech.univ.gda.pl
The cgtA gene product is a member of the subfamily of small GTP-binding proteins that have been identified in diverse organisms ranging from bacteria to humans. In bacteria that sporulate or display another special developmental programme, this gene (referred to as cgtA, obg or yhbZ) appears to be involved in the regulation of these processes. However, this gene has also been found to be essential in all bacterial species investigated to date, although its role in bacteria that do not sporulate and do not undergo a specific development remains unknown. Here the authors characterize a Vibrio harveyi mutant bearing a transposon insertion into the cgtA gene. This mutant reveals a multiple phenotype: it grows more slowly than the wild-type strain in a rich medium; its growth is completely inhibited in minimal media; its survival in 3% NaCl is dramatically reduced; it is very sensitive to UV irradiation; it is more susceptible to mutation upon treatment with different mutagens; its luminescence is decreased; its quorum-sensing regulation is less effective than in the wild-type strain; and the elongated shape of the mutant cells may suggest problems with the regulation of cell division and/or DNA replication. These defects in diverse cellular processes found in the insertional cgtA mutant of V. harveyi indicate that in a bacterium that does not sporulate and does not display other special development programmes, the CgtA protein is involved in the regulation of many crucial biochemical reactions, possibly at the stage of signal transduction.
Keywords: cgtA gene, GTP-binding protein, bioluminescence, signal transduction
Abbreviations: DAPI, 4',6-diamidino-2-phenylindole
The GenBank accession number for the sequence reported in this paper is AF247677.
This article has been cited by other articles:
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  A. E. Sikora, R. Zielke, K. Datta, and J. R. Maddock The Vibrio harveyi GTPase CgtAV Is Essential and Is Associated with the 50S Ribosomal Subunit J. Bacteriol., February 1, 2006; 188(3): 1205 - 1210. [Abstract] [Full Text] [PDF]
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B. Lin, D. A. Thayer, and J. R. Maddock The Caulobacter crescentus CgtAC Protein Cosediments with the Free 50S Ribosomal Subunit J. Bacteriol., January 15, 2004; 186(2): 481 - 489. [Abstract] [Full Text] [PDF]
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 R. Zielke, A. Sikora, R. Dutkiewicz, G. Wegrzyn, and A. Czyz Involvement of the cgtA gene function in stimulation of DNA repair in Escherichia coli and Vibrio harveyi Microbiology, July 1, 2003; 149(7): 1763 - 1770. [Abstract] [Full Text] [PDF]
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 D. S. Millikan and E. G. Ruby Alterations in Vibrio fischeri Motility Correlate with a Delay in Symbiosis Initiation and Are Associated with Additional Symbiotic Colonization Defects Appl. Envir. Microbiol., May 1, 2002; 68(5): 2519 - 2528. [Abstract] [Full Text] [PDF]
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