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Genetics and Molecular Biology |
Division of Industrial Microbiology, Department of Food Technology and Nutritional Sciences, Wageningen Agricultural University, PO Box 8129, 6700 EV Wageningen, The Netherlands1
Author for correspondence: Jasper Kieboom. Tel: +31 317 484412. Fax: +31 317 484978. e-mail: jasper.kieboom{at}imb.ftns.wau.nl
The authors previously described srpABC, an operon involved in proton-dependent solvent efflux in the solvent-tolerant Pseudomonas putida S12. Recently, it was shown that organic solvents and not antibiotics induce this operon. In the present study, the authors characterize a new efflux pump, designated ArpABC, on the basis of two isolated chloramphenicol-sensitive transposon mutants. The arpABC operon is involved in the active efflux of multiple antibiotics, such as tetracycline, chloramphenicol, carbenicillin, streptomycin, erythromycin and novobiocin. The deduced amino acid sequences encoded by the three genes involved show a striking resemblance to proteins of the resistance/nodulation/cell division family, which are involved in both organic solvent and multiple drug efflux. These findings demonstrate that ArpABC is highly homologous to the MepABC and TtgABC efflux systems for organic solvents and multiple antibiotics. However, ArpABC does not contribute to organic solvent tolerance in P. putida S12 but is solely involved in multidrug resistance.
Keywords: solvent tolerance, multidrug resistance, efflux
Abbreviations: RND family, resistance/nodulation/cell division family
The GenBank accession number for the arp sequence is AF183959.
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