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Microbiology (2001), 147, 3083-3092.
© 2001 Society for General Microbiology

Genetics and Molecular Biology

A polyketide biosynthetic gene cluster from Streptomyces antibioticus includes a LysR-type transcriptional regulator

Victoria Colomboa,1, Maria Fernández-de-Heredia1 and Francisco Malpartida1

Centro Nacional de Biotecnologia, Campus de la Universidad Autónoma de Madrid, 28049 Cantoblanco, Madrid, Spain1

Author for correspondence: Francisco Malpartida. Tel: +34 91 5854548. Fax: +34 91 5854506. e-mail: fmalpart{at}cnb.uam.es

In the search for Type II polyketide synthases (PKSs) a DNA fragment was isolated from Streptomyces antibioticus ATCC 11891 (a producer of oleandomycin). DNA sequencing of the cloned fragment revealed six complete ORFs whose deduced products showed similarities to those of other genes known to be involved in polyketide biosynthesis. Several S. coelicolor strains mutated in different steps of actinorhodin biosynthesis (actI, actIII, actVA and actVII) were complemented by the cloned genes, suggesting that the isolated genes encode an aromatic polyketide of unknown structure and function. The cluster also contains a putative LysR-type transcriptional regulator (ORF0), which controls PKS gene expression in a heterologous host. DNA binding assays and transcriptional analysis suggest that the pathway-specific regulator for actinorhodin biosynthesis (actII-ORF4) is also involved in the expression of the cloned PKS in the host strain.

Keywords: actII-ORF4, actinorhodin, antibiotic, heterologous complementation, regulation

Abbreviations: PKS, polyketide synthase

The GenBank accession number for the sequence reported in this paper is Y19177.

a Present address: Department of Molecular Microbiology, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.






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Copyright © 2001 Society for General Microbiology.