| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Physiology and Growth |
Laboratoire d’Ingénierie des Protéines et Contrôle Métabolique, Institut Jacques Monod, Tour 43, Université Paris 7/Paris 6, 2 place Jussieu, 75251 Paris cedex 05, France1
University of Pennsylvania, Department of Medicine, Division of Hematology/Oncology, BRBII Room 731, 431 Curie Blvd, Philadelphia, PA 19104, USA2
Author for correspondence: Emmanuel Lesuisse. Tel: +33 1 44 27 63 56. Fax: +33 1 44 27 57 16. e-mail: lesuisse{at}ijm.jussieu.fr
The non-reductive uptake of several siderophores (ferrioxamine B, ferrichrome, triacetylfusarinine C and ferricrocin) by various strains of Saccharomyces cerevisiae was studied. Several aspects of siderophore transport were examined, including specificity of transport, regulation of transport and intracellular localization of the ferri-siderophores. Ferrioxamine B was taken up preferentially via the products of the SIT1 gene and triacetylfusarinine C by the TAF1 gene product, but the specificity was not absolute. Ferrichrome and ferricrocin uptake was not dependent on a single major facilitator superfamily (MFS) gene product. The apparent specificity of transport was strongly dependent on the genetic background of the cells. Non-reductive uptake of siderophores was induced under more stringent conditions (of iron deprivation) than was the reductive uptake of ferric citrate. Regulation of transport depended on the transcriptional factors Aft1 and Tup1/Ssn6. Cells disrupted for the TUP1 or SSN6 genes were constitutively derepressed for the uptake of ferrichrome, ferricrocin or ferrioxamine B, but not for the uptake of triacetylfusarinine C. Cells bearing the AFT1up mutation accumulated large amounts of ferric siderophores. Intracellular decomplexation of the siderophores occurred when transcription of the AFT1up gene was repressed. Ferrioxamine B and ferrichrome seemed to accumulate in an endosomal compartment, as shown by biochemical studies and by confocal microscopy study of cells loaded with a fluorescent derivative of ferrichrome. Endocytosis was, however, not involved in the non-reductive uptake of siderophores.
Keywords: iron, siderophore, yeast, Saccharomyces cerevisiae
Abbreviations: BPS, bathophenanthrolinedisulfonate; FC, ferricrocin; FCH, ferrichrome; FOB, ferrioxamine B, mesylate form (Desferal); MFS, major facilitator superfamily; TAF, triacetylfusarinine C
This article has been cited by other articles:
|
|
 |
|
  C. C. Philpott and O. Protchenko Response to Iron Deprivation in Saccharomyces cerevisiae Eukaryot. Cell, January 1, 2008; 7(1): 20 - 27. [Full Text] [PDF]
|
|
|
|
 |
|
 M. Miethke and M. A. Marahiel Siderophore-Based Iron Acquisition and Pathogen Control Microbiol. Mol. Biol. Rev., September 1, 2007; 71(3): 413 - 451. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Tangen, W. H. Jung, A. P. Sham, T. Lian, and J. W. Kronstad The iron- and cAMP-regulated gene SIT1 influences ferrioxamine B utilization, melanization and cell wall structure in Cryptococcus neoformans Microbiology, January 1, 2007; 153(1): 29 - 41. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yamanaka, H. Oikawa, H.-o. Ogawa, K. Hosono, F. Shinmachi, H. Takano, S. Sakuda, T. Beppu, and K. Ueda Desferrioxamine E produced by Streptomyces griseus stimulates growth and development of Streptomyces tanashiensis Microbiology, September 1, 2005; 151(9): 2899 - 2905. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Courel, S. Lallet, J.-M. Camadro, and P.-L. Blaiseau Direct Activation of Genes Involved in Intracellular Iron Use by the Yeast Iron-Responsive Transcription Factor Aft2 without Its Paralog Aft1 Mol. Cell. Biol., August 1, 2005; 25(15): 6760 - 6771. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Lesuisse, S. A. B. Knight, M. Courel, R. Santos, J.-M. Camadro, and A. Dancis Genome-Wide Screen for Genes With Effects on Distinct Iron Uptake Activities in Saccharomyces cerevisiae Genetics, January 1, 2005; 169(1): 107 - 122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Puig, M. Lau, and D. J. Thiele Cti6 Is an Rpd3-Sin3 Histone Deacetylase-associated Protein Required for Growth under Iron-limiting Conditions in Saccharomyces cerevisiae J. Biol. Chem., July 16, 2004; 279(29): 30298 - 30306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Roberts, A. J. Pierson, Z. Panaviene, and E. L. Walker Yellow Stripe1. Expanded Roles for the Maize Iron-Phytosiderophore Transporter Plant Physiology, May 1, 2004; 135(1): 112 - 120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Znaidi, B. Pelletier, Y. Mukai, and S. Labbe The Schizosaccharomyces pombe Corepressor Tup11 Interacts with the Iron-responsive Transcription Factor Fep1 J. Biol. Chem., March 5, 2004; 279(10): 9462 - 9474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Pelletier, J. Beaudoin, C. C. Philpott, and S. Labbe Fep1 represses expression of the fission yeast Schizosaccharomyces pombe siderophore-iron transport system Nucleic Acids Res., August 1, 2003; 31(15): 4332 - 4344. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Moore, Y. Kim, and C. C. Philpott The mechanism of ferrichrome transport through Arn1p and its metabolism in Saccharomyces cerevisiae PNAS, May 13, 2003; 100(10): 5664 - 5669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. La Fontaine, J. M. Quinn, S. S. Nakamoto, M. D. Page, V. Gohre, J. L. Moseley, J. Kropat, and S. Merchant Copper-Dependent Iron Assimilation Pathway in the Model Photosynthetic Eukaryote Chlamydomonas reinhardtii Eukaryot. Cell, October 1, 2002; 1(5): 736 - 757. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. M. Waters and D. J. Eide Combinatorial Control of Yeast FET4 Gene Expression by Iron, Zinc, and Oxygen J. Biol. Chem., September 6, 2002; 277(37): 33749 - 33757. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Heymann, M. Gerads, M. Schaller, F. Dromer, G. Winkelmann, and J. F. Ernst The Siderophore Iron Transporter of Candida albicans (Sit1p/Arn1p) Mediates Uptake of Ferrichrome-Type Siderophores and Is Required for Epithelial Invasion Infect. Immun., September 1, 2002; 70(9): 5246 - 5255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-J. Hu, C. Bai, X.-D. Zheng, Y.-M. Wang, and Y. Wang Characterization and Functional Analysis of the Siderophore-Iron Transporter CaArn1p in Candida albicans J. Biol. Chem., August 16, 2002; 277(34): 30598 - 30605. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. R. Emerson, M. E. Nau, R. K. Martin, D. E. Kyle, M. Vahey, and D. F. Wirth Relationship between Chloroquine Toxicity and Iron Acquisition in Saccharomyces cerevisiae Antimicrob. Agents Chemother., March 1, 2002; 46(3): 787 - 796. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. B. Knight, E. Lesuisse, R. Stearman, R. D. Klausner, and A. Dancis Reductive iron uptake by Candida albicans: role of copper, iron and the TUP1 regulator Microbiology, January 1, 2002; 148(1): 29 - 40. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Timmerman and J. P. Woods Potential Role for Extracellular Glutathione-Dependent Ferric Reductase in Utilization of Environmental and Host Ferric Compounds by Histoplasma capsulatum Infect. Immun., December 1, 2001; 69(12): 7671 - 7678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Ardon, H. Bussey, C. Philpott, D. M. Ward, S. Davis-Kaplan, S. Verroneau, B. Jiang, and J. Kaplan Identification of a Candida albicans Ferrichrome Transporter and Its Characterization by Expression in Saccharomyces cerevisiae J. Biol. Chem., November 9, 2001; 276(46): 43049 - 43055. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-L. Blaiseau, E. Lesuisse, and J.-M. Camadro Aft2p, a Novel Iron-regulated Transcription Activator That Modulates, with Aft1p, Intracellular Iron Use and Resistance to Oxidative Stress in Yeast J. Biol. Chem., August 31, 2001; 276(36): 34221 - 34226. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
