| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Genetics and Molecular Biology |
Center for Adaptation Genetics and Drug Resistance, and Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA1
Author for correspondence: Stuart B. Levy. Tel: +1 617 636 6764. Fax: +1 617 636 0458. e-mail: stuart.levy{at}tufts.edu
The locations of two mutations that prevent adhesion of Pseudomonas fluorescens Pf0-1 to sand columns and seeds (adn, adhesion) were identified. Both lie in a single gene showing homology to the NtrC/NifA family of transcription activators. The predicted 55 kDa protein encoded by adnA is most closely related to activators involved in expression of flagellar proteins, consistent with the lack of flagella in adnA strains. Constitutive adnA expression restored motility and adhesion to an adnA strain, demonstrating that the observed phenotypes are due to lack of AdnA and not a consequence of other mutations or polar effects of mutations in adnA on other genes.
Keywords: adhesion, flagella, transcription regulation
The GenBank accession number for the sequence reported in this paper is AF312695.
a Present address: Lawrence Livermore National Laboratories, 7000 East Avenue, Livermore, CA 94550, USA.
b Present address: Millennium Pharmaceuticals Inc., 640 Memorial Drive, Cambridge, MA 02139, USA.
c Present address: University of Massachusetts-Dartmouth, Department of Biology, Dartmouth, MA 02747, USA.
d Present address: Slippery Rock University, Department of Biology, Slippery Rock, PA 16057, USA.
This article has been cited by other articles:
|
|
 |
|
  W. Kim and S. B. Levy Increased Fitness of Pseudomonas fluorescens Pf0-1 Leucine Auxotrophs in Soil Appl. Envir. Microbiol., June 15, 2008; 74(12): 3644 - 3651. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Redondo-Nieto, J. Lloret, J. Larenas, E. Barahona, A. Navazo, F. Martinez-Granero, S. Capdevila, R. Rivilla, and M. Martin Transcriptional Organization of the Region Encoding the Synthesis of the Flagellar Filament in Pseudomonas fluorescens J. Bacteriol., June 1, 2008; 190(11): 4106 - 4109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Leonard, J. Ferooz, V. Haine, I. Danese, D. Fretin, A. Tibor, S. de Walque, X. De Bolle, and J.-J. Letesson FtcR Is a New Master Regulator of the Flagellar System of Brucella melitensis 16M with Homologs in Rhizobiaceae J. Bacteriol., January 1, 2007; 189(1): 131 - 141. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Silby and S. B. Levy Use of In Vivo Expression Technology To Identify Genes Important in Growth and Survival of Pseudomonas fluorescens Pf0-1 in Soil: Discovery of Expressed Sequences with Novel Genetic Organization J. Bacteriol., November 1, 2004; 186(21): 7411 - 7419. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Capdevila, F. M. Martinez-Granero, M. Sanchez-Contreras, R. Rivilla, and M. Martin Analysis of Pseudomonas fluorescens F113 genes implicated in flagellar filament synthesis and their role in competitive root colonization Microbiology, November 1, 2004; 150(11): 3889 - 3897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Robleto, I. Lopez-Hernandez, M. W. Silby, and S. B. Levy Genetic Analysis of the AdnA Regulon in Pseudomonas fluorescens: Nonessential Role of Flagella in Adhesion to Sand and Biofilm Formation J. Bacteriol., January 15, 2003; 185(2): 453 - 460. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
