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Microbiology 147 (2001), 611-619
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Microbiology (2001), 147, 611-619.
© 2001 Society for General Microbiology

Environmental Microbiology

Sequence variation in dichloromethane dehalogenases/glutathione S-transferases

Stéphane Vuilleumier1, Nikola Ivos1, Mariangela Dean2 and Thomas Leisinger1

Institut für Mikrobiologie, ETH Zürich, Schmelzbergstraße 7, CH-8092 Zürich, Switzerland1
Dipartimento di Biochimica e Biologia Molecolare, Università degli Studi, 44100 Ferrara, Italy2

Author for correspondence: Stéphane Vuilleumier. Tel: +41 1 632 33 57. Fax: +41 1 632 11 48. e-mail: svuilleu{at}micro.biol.ethz.ch

Dichloromethane dehalogenase/glutathione S-transferase allows methylotrophic bacteria to grow with dichloromethane (DCM), a predominantly man-made compound. Bacteria growing with DCM by virtue of this enzyme have been readily isolated in the past. So far, the sequence of the dcmA gene encoding DCM dehalogenase has been determined for Methylobacterium dichloromethanicum DM4 and Methylophilus sp. DM11. DCM dehalogenase genes closely related to that of strain DM4 were amplified by PCR and cloned from total DNA from 14 different DCM-degrading strains, enrichment cultures and sludge samples from wastewater treatment plants. In total, eight different sequences encoding seven different protein sequences were obtained. Sequences of different origin were identical in several instances. Sequence variation was limited to base substitutions; strikingly, 16 of the 19 substitutions in the dcmA gene itself encoded amino acids that were different from those of the DM4 sequence. The kinetic parameters kcat and Km, the pH optimum and the stability of representative DCM dehalogenase variants were investigated, revealing minor differences between the properties of DCM dehalogenases related to that from strain DM4.

Keywords: dichloromethane, dehalogenase, glutathione S-transferase

Abbreviations: CAH, chlorinated aliphatic hydrocarbon; DCM, dichloromethane; GST, glutathione S-transferase

The GenBank accession numbers for the sequences determined in this work are AJ271131–38 (see text for details).




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M. F. Kayser and S. Vuilleumier
Dehalogenation of Dichloromethane by Dichloromethane Dehalogenase/Glutathione S-Transferase Leads to Formation of DNA Adducts
J. Bacteriol., September 1, 2001; 183(17): 5209 - 5212.
[Abstract] [Full Text] [PDF]


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