| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Genetics and Molecular Biology |
EC Slater Institute, BioCentrum, University of Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands1
TNO Voeding, Department of Applied Microbiology and Gene Technology, PO box 360 3700 AJ Zeist, The Netherlands2
Author for correspondence: Peter H. Pouwels (TNO Voeding). Tel: +31 30 6944 924. Fax: +31 30 6944 466. e-mail: Pouwels{at}voeding.tno.nl
The role of the Lactobacillus pentosus phosphoenolpyruvate:mannose phosphotransferase system (mannose PTS) in sugar transport and control of sugar utilization was investigated. Growth experiments and measurements of PEP-dependent phosphorylation of sugars, of sugar transport and of catabolic enzyme activity were performed, to compare a wild-type strain with an EIIBMan mutant, LPE6, and a ccpA mutant, LPE4. Fructose uptake in wild-type bacteria demonstrated the presence of two fructose-specific PTSs: a high-affinity system, EIIFru (Km=52 µM) which is inducible by fructose, and a low-affinity system (Km=300 µM). The latter system was lacking in LPE6 and therefore corresponds to EIIMan. LPE6 was unable to phosphorylate glucose, mannose, N-acetylglucosamine and 2-deoxyglucose in a PEP-dependent reaction, indicating that these sugars are substrates of EIIMan. Transport and phosphorylation of these compounds was the same in LPE4 and in wild-type bacteria, although growth of LPE4 on these sugars was impaired. In wild-type bacteria and in LPE4 the activity of EIIFru was lowered by the presence of EIIMan substrates in the growth medium, but this decrease was not observed in LPE6. These results indicate that EIIMan but not CcpA regulates the synthesis of EIIFru. Mutations in EIIMan or CcpA resulted in a relief of catabolite repression exerted by EIIMan substrates on the activity of ß-galactosidase and ß-glucosidase, indicating that EIIMan and CcpA are important components in catabolite repression in L. pentosus. Fructose-mediated repression of these two enzymes appeared to be correlated with the activity of EIIFru.
Keywords: mannose PTS, fructose PTS, CcpA, sugar transport, regulation
Abbreviations: 2DG, 2-deoxyglucose; CR, catabolite repression; mannose PTS, phosphoenolpyruvate:mannose phosphotransferase system; PEP, phosphoenolpyruvate
a Present address: Institut National de la Recherche Agronomique, Unité Flore Lactique et Environnement Carné, CRJ, Domaine de Vilvert, 78350 Jouy-en-Josas, France.
This article has been cited by other articles:
|
|
 |
|
  J.-H. Kim, S. P. Shoemaker, and D. A. Mills Relaxed control of sugar utilization in Lactobacillus brevis Microbiology, April 1, 2009; 155(4): 1351 - 1359. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Goh, P. F. Ong, K. P. Song, T. V. Riley, and B. J. Chang The complete genome sequence of Clostridium difficile phage {phi}C2 and comparisons to {phi}CD119 and inducible prophages of CD630 Microbiology, March 1, 2007; 153(3): 676 - 685. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Arous, C. Buchrieser, P. Folio, P. Glaser, A. Namane, M. Hebraud, and Y. Hechard Global analysis of gene expression in an rpoN mutant of Listeria monocytogenes Microbiology, May 1, 2004; 150(5): 1581 - 1590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Vadyvaloo, J. L. Snoep, J. W. Hastings, and M. Rautenbach Physiological implications of class IIa bacteriocin resistance in Listeria monocytogenes strains Microbiology, February 1, 2004; 150(2): 335 - 340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Lessard, A. Cochu, J.-D. Lemay, D. Roy, K. Vaillancourt, M. Frenette, S. Moineau, and C. Vadeboncoeur Phosphorylation of Streptococcus salivarius Lactose Permease (LacS) by HPr(His~P) and HPr(Ser-P)(His~P) and Effects on Growth J. Bacteriol., December 1, 2003; 185(23): 6764 - 6772. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Cochu, C. Vadeboncoeur, S. Moineau, and M. Frenette Genetic and Biochemical Characterization of the Phosphoenolpyruvate:Glucose/Mannose Phosphotransferase System of Streptococcus thermophilus Appl. Envir. Microbiol., September 1, 2003; 69(9): 5423 - 5432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Abranches, Y.-Y. M. Chen, and R. A. Burne Characterization of Streptococcus mutans Strains Deficient in EIIABMan of the Sugar Phosphotransferase System Appl. Envir. Microbiol., August 1, 2003; 69(8): 4760 - 4769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gravesen, M. Ramnath, K. B. Rechinger, N. Andersen, L. Jansch, Y. Hechard, J. W. Hastings, and S. Knochel High-level resistance to class IIa bacteriocins is associated with one general mechanism in Listeria monocytogenes Microbiology, August 1, 2002; 148(8): 2361 - 2369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Posthuma, R. Bader, R. Engelmann, P. W. Postma, W. Hengstenberg, and P. H. Pouwels Expression of the Xylulose 5-Phosphate Phosphoketolase Gene, xpkA, from Lactobacillus pentosus MD363 Is Induced by Sugars That Are Fermented via the Phosphoketolase Pathway and Is Repressed by Glucose Mediated by CcpA and the Mannose Phosphoenolpyruvate Phosphotransferase System Appl. Envir. Microbiol., February 1, 2002; 68(2): 831 - 837. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
