Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 147 (2001), 727-733
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kok, M.
Right arrow Articles by Pechère, J.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kok, M.
Right arrow Articles by Pechère, J.-C.
Agricola
Right arrow Articles by Kok, M.
Right arrow Articles by Pechère, J.-C.
Microbiology (2001), 147, 727-733.
© 2001 Society for General Microbiology

Pathogenicity and Medical Microbiology

Salmonella typhimurium thyA mutants fail to grow intracellularly in vitro and are attenuated in mice

Menno Kok1, Elisabeth Bühlmann1 and Jean-Claude Pechère1

Département de Génétique et Microbiologie, Faculté de Médecine, Université de Genève, 1 Rue Michel Servet, 1211 Genève, Switzerland1

Author for correspondence: Menno Kok. Tel: +41 22 7025652. Fax: +41 22 7025702. e-mail: menno.kok{at}medecine.unige.ch

Salmonella typhimurium ATCC14028 readily multiplies in professional phagocytes in vitro and is highly virulent in mice. Mutants lacking thymidylate synthase activity (thyA) were isolated and shown to be strictly dependent on thymidine monophosphate precursors in the growth medium. The thyA mutants were found to be virtually incapable of intracellular growth and survival in vitro, both in macrophage-like cell line P338D1 and in the human epithelial cell line Hep-2, and their virulence was impaired in BALB/c mice. Intraperitoneal immunization of mice with two doses of live S. typhimurium thyA provided protection against a challenge with 103 times the 50% lethal dose of the virulent parent strain.

Keywords: Salmonella, virulence, thymidylate synthase

Abbreviations: i.p., intraperitoneal; HBSS, Hanks basic salt solution; TMP, thymidine monophosphate




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Rollenhagen, M. Sorensen, K. Rizos, R. Hurvitz, and D. Bumann
Antigen selection based on expression levels during infection facilitates vaccine development for an intracellular pathogen
PNAS, June 8, 2004; 101(23): 8739 - 8744.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2001 Society for General Microbiology.