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Genetics and Molecular Biology |
Microbiology Unit, Department of Biochemistry, South Parks Road, Oxford OX1 3QU, UK1
Author for correspondence: Keith Dyke. Tel: +44 1865 275293. Fax: +44 1865 275297. e-mail: kdyke{at}bioch.ox.ac.uk
The precise start points for transcription of the blaZ and of the blaRI/blaI genes of the Staphylococcus aureus ß-lactamase operon have been determined by primer extension analysis. Consequently the overlapping promoter sequences were deduced. Northern blots showed that the synthesis of the 2100 nt mRNA from blaRI is inducible and that a blaI probe hybridized to the same mRNA as the blaRI probe. The gene cat, encoding chloramphenicol acetyltransferase, was fused separately to the blaZ and blaRI/blaI promoters, and used to compare their strengths. The promoter for blaZ is about six times stronger than that for blaRI/blaI and the synthesis of chloramphenicol acetyltransferase from both promoters is inducible, supporting the results from the Northern blots.
Keywords: penicillin resistance, promoter strength, transcription
Abbreviations: CAT, chloramphenicol acetyltransferase; CBAP, 2-(2'-carboxyphenyl)-benzoyl-6-aminopenicillanic acid; MRSA, methicillin-resistant Staphylococcus aureus
a Present address: Department of Molecular Biology and Biotechnology, Firth Court, University of Sheffield, Western Bank, Sheffield S10 2TN, UK.
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