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Surface exposure and protease insensitivity of Borrelia burgdorferi Erp (OspEF-related) lipoproteins

Department of Microbiology and Immunology, University of Kentucky College of Medicine, MS 415 Chandler Medical Center, Lexington, KY 40536-0298, USA¹
Microscopy Branch, Rocky Mountain Laboratories, NIAID, National Institutes of Health, 903 South 4th St, Hamilton, MT 59840, USA²
Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, PO Box 2087, Foothills Campus, Fort Collins, CO 80522, USA³
Department of Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523-1671, USA⁴

Author for correspondence: Brian Stevenson. Tel: +1 859 257 9358. Fax: +1 859 257 8994. e-mail: bstev0{at}pop.uky.edu

Borrelia burgdorferi can encode numerous lipoproteins of the Erp family. Although initially described as outer surface proteins, the technique used in that earlier study has since been demonstrated to disrupt bacterial membranes and allow labelling of subsurface proteins. Data are now presented from additional analyses indicating that Erp proteins are indeed surface exposed in the outer membrane. Surface localization of these infection-associated proteins indicates the potential for interactions of Erp proteins with vertebrate tissues. Some Erp proteins were resistant to in situ digestion by certain proteases, suggesting that those proteins fold in manners which hide protease cleavage sites, or that they interact with other protective membrane components. Additionally, cultivation of B. burgdorferi in the presence of antibodies directed against Erp proteins inhibited bacterial growth.

Keywords: spirochaete, immunofluourescence, Erp proteins, bacterial surface proteins, protease resistance, Lyme disease

Abbreviations: IFA, immunofluorescent antibody

^a Present address: Dept. of Molecular Biology, University of Wyoming, Laramie, WY 82071, USA.

^b Present address: Centocor Inc., 200 Great Valley Parkway, Malvern, PA 19355, USA.

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