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Microbiology 147 (2001), 965-972
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Microbiology (2001), 147, 965-972.
© 2001 Society for General Microbiology

Genetics and Molecular Biology

The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions

Sylvia Franke1, Gregor Grass1 and Dietrich H. Nies1

Institut für Mikrobiologie der Martin-Luther-Universität Halle-Wittenberg, Kurt-Mothes-Str. 3, 06099 Halle, Germany1

Author for correspondence: Dietrich H. Nies. Tel: +49 345 55 26352. Fax: +49 345 55 27010. e-mail: d.nies{at}mikrobiologie.uni-halle.de

Transcription of the ybcZ–ylcA ylcBCD–ybdE region of the Escherichia coli K38 chromosome was analysed by Northern RNA–DNA hybridization, RT-PCR and primer extension. Transcription of a dicistronic ybcZ–ylcA mRNA and a tetracistronic ylcBCD–ybdE mRNA was induced by silver and was initiated from the sigma-70 promoters ylcAp and ylcBp. Expression of ß-galactosidase activity from a {Phi}(ylcBp–lacZ) operon fusion was also induced by Ag+ and Cu2+, but not by Zn2+. In-frame deletion of ybdE from the chromosome yielded a silver-sensitive E. coli mutant strain which did not differ in its copper resistance from its wild-type strain. On the other hand, deletion of the copA gene for the copper-exporting P-type ATPase CopA resulted in copper sensitivity, but not in silver sensitivity. A {Delta}ybdE {Delta}copA double mutant strain behaved towards copper as the {Delta}copA strain and towards silver as the {Delta}ybdE strain. Thus, in E. coli, the YlcBCD–YbdE system may be involved in silver- but not in copper resistance, and CopA may be involved in copper- but not in silver resistance.

Keywords: heavy metal resistance, cation efflux, silver resistance, RND family




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