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Genetics and Molecular Biology |
Department of Microbiology, Eastman Dental Institute for Oral Health Care Sciences, University College London, University of London, 256 Gray’s Inn Road, London WC1X 8LD, UK1
Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Vic 3800, Australia2
Author for correspondence: Peter Mullany. Tel: +44 20 7915 1223. Fax: +44 20 7915 1127. e-mail: p.mullany{at}eastman.ucl.ac.uk
Comparative analysis of the conjugative transposons Tn5397 from Clostridium difficile and Tn916 from Enterococcus faecalis, and the CW459tet(M) element from Clostridium perfringens, has revealed that these tetracycline-resistance elements are closely related. All three elements contain the tet(M) resistance gene and have sequence similarity throughout their central region. However, they have very different integration/excision modules. Instead of the int and xis genes that are found in Tn916, Tn5397 has a large resolvase gene, tndX. The C. perfringens element encodes the putative Int459 protein, which is a member of the integrase family of site-specific recombinases but is not closely related to Int from Tn916. Based on these studies it is concluded that the clostridial elements have a modular genetic organization and were derived independently from distinct mobile genetic elements.
Keywords: conjugative transposons, gene transfer, antibiotic resistance, mobile elements
The GenBank accession numbers for the sequences in this paper are AF333235 (Tn5397) and AF329848 [part of CW459tet(M)].
a These authors contributed equally to the work.
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