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Genetics and Molecular Biology |
Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK1
Author for correspondence: Simon J. Foster. Tel: +44 114 222 4411. Fax: +44 114 272 8697. e-mail: s.foster{at}sheffield.ac.uk
A putative operon encoding a probable zinc-responsive regulatory element (zur) and components of an ABC-type transporter (mreA mreB) have been characterized in Staphylococcus aureus. The zur gene was inactivated but apparently this did not alter Zn2+ uptake. Expression of mreAB zur is at a low level under a range of ion conditions. To allow inducible expression of the operon, a construct was made placing it under the control of the IPTG-inducible Pspac promoter. Using this approach, it was shown that zur is able to repress expression of the entire operon in a Zn2+-dependent manner, and that mreA and mreB are likely to be involved in high-affinity ion uptake. zur has no apparent role in pathogenicity in a lesion model of S. aureus infection.
Keywords: metals, transcription, zinc, fur, regulation
The GenBank accession number for the sequence reported in this paper is AF101263.
a Present address: Division of Infectious Disease, St George’s Hospital Medical School, Cranmer Terrace, London SW17 0RE, UK.
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