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A peptidorhamnomannan from the mycelium of Pseudallescheria boydii is a potential diagnostic antigen of this emerging human pathogen

Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Ilha do Fundão, 21941-970, Rio de Janeiro, RJ, Brazil¹
National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK²
Instituto Biomédico, Universidade do Rio de Janeiro (UNIRIO), Rio de Janeiro, Brazil³
Disciplina de Biologia Celular, Universidade Federal de São Paulo, São Paulo, Brazil⁴

Author for correspondence: Eliana Barreto Bergter. Tel: +55 21 590 3093. Fax: +55 21 5608344. e-mail: immgbel{at}microbio.ufrj.br

The ascomycete Pseudallescheria boydii is an emerging human pathogen frequently found in soil and polluted water. A peptidopolysaccharide antigen has been isolated from mycelial forms of P. boydii, and characterized using chemical and immunological methods. Monosaccharide composition, methylation analysis, and ¹H- and ¹³C-NMR spectra indicated the presence of a rhamnomannan with a structure distinct from those of similar components isolated from other fungi, containing Rhap(13)Rhap epitopes on side chains which may be linked (13) to (16)-linked mannose. The peptidorhamnomannan from P. boydii reacted poorly with an antiserum raised against whole cells of Sporothrix schenckii and strongly with one against P. boydii hyphae. These characteristics and immunological differences suggest that this major rhamnose-containing antigen of P. boydii may be useful for the specific diagnosis of infections attributable to this fungus.

Keywords: fungal pathogen, mycelium, peptidopolysaccharide, ¹H- and ¹³C-nuclear magnetic resonance

Abbreviations: COSY, correlated spectroscopy; DEPT, distortionless enhancement by polarization transfer; PAS, periodic acid/Schiff; TSP, trimethylsilylpropionic acid sodium salt

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