| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Environmental Microbiology |
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK1
Max-Planck-Institut für Marine Mikrobiologie, Bremen, Germany2
Unité de Physiologie Microbienne (CNRS URA 2172), Institut Pasteur, Paris, France3
The H. Steinitz Marine Biology Laboratory, InterUniversity Institute of Eilat, Eilat, Israel4
Author for correspondence: David J. Scanlan. Tel: +44 24 76 528363. Fax: +44 24 76 523701. e-mail: dscanlan{at}bio.warwick.ac.uk
An in situ hybridization method was applied to the identification of marine cyanobacteria assignable to the genus Prochlorococcus using horseradish-peroxidase-labelled 16S rRNA-targeted oligonucleotide probes in combination with tyramide signal amplification (TSA). With this method very bright signals were obtained, in contrast to hybridizations with oligonucleotides monolabelled with fluorochromes, which failed to give positive signals. Genotype-specific oligonucleotides for high light (HL)- and low light (LL)-adapted members of this genus were identified by 16S rRNA sequence analyses and their specificities confirmed in whole-cell hybridizations with cultured strains of Prochlorococcus marinus Chisholm et al., 1992 , Prochlorococcus sp. and Synechococcus sp. In situ hybridization of these genotype-specific probes to field samples from stratified water bodies collected in the North Atlantic Ocean and the Red Sea allowed a rapid assessment of the abundance and spatial distribution of HL- and LL-adapted Prochlorococcus. In both oceanic regions the LL-adapted Prochlorococcus populations were localized in deeper water whereas the HL-adapted Prochlorococcus populations were not only distinct in each region but also exhibited strikingly different depth distributions, HLI being confined to shallow water in the North Atlantic, in contrast to HLII, which was present throughout the water column in the Red Sea.
Keywords: cyanobacteria, Red Sea, oligonucleotide probes, 16S rRNA, TSA
Abbreviations: DAPI, 4’,6-diamidino-2-phenylindole; HL, high light; HRP, horseradish peroxidase; LL, low light; TETON, 4-(1,4,7,10-tetra-oxadecyl)-1-naphthol; TSA, tyramide signal amplification
The GenBank accession numbers for the sequences reported in this paper are AF311217 (RCC278, EQPAC1), AF311218 (RCC277, NATL1MIT), AF311219 (RCC280, NATL2B), AF311220 (RCC264, TAK9803-2), AF311291 (WH7803), AF311292 (WH8018) and AF311293 (WH8103).
This article has been cited by other articles:
|
|
 |
|
  M. V. Zubkov Photoheterotrophy in marine prokaryotes J. Plankton Res., June 18, 2009; (2009) fbp043v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Scanlan, M. Ostrowski, S. Mazard, A. Dufresne, L. Garczarek, W. R. Hess, A. F. Post, M. Hagemann, I. Paulsen, and F. Partensky Ecological Genomics of Marine Picocyanobacteria Microbiol. Mol. Biol. Rev., June 1, 2009; 73(2): 249 - 299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Tolonen, G. B. Liszt, and W. R. Hess Genetic Manipulation of Prochlorococcus Strain MIT9313: Green Fluorescent Protein Expression from an RSF1010 Plasmid and Tn5 Transposition Appl. Envir. Microbiol., December 1, 2006; 72(12): 7607 - 7613. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Steglich, M. Futschik, T. Rector, R. Steen, and S. W. Chisholm Genome-Wide Analysis of Light Sensing in Prochlorococcus J. Bacteriol., November 15, 2006; 188(22): 7796 - 7806. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Martiny, M. L. Coleman, and S. W. Chisholm Phosphate acquisition genes in Prochlorococcus ecotypes: Evidence for genome-wide adaptation PNAS, August 15, 2006; 103(33): 12552 - 12557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Tobe, G. Eller, and L. K. Medlin Automated detection and enumeration for toxic algae by solid-phase cytometry and the introduction of a new probe for Prymnesium parvum (Haptophyta: Prymnesiophyceae) J. Plankton Res., July 1, 2006; 28(7): 643 - 657. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Coleman, M. B. Sullivan, A. C. Martiny, C. Steglich, K. Barry, E. F. DeLong, and S. W. Chisholm Genomic islands and the ecology and evolution of Prochlorococcus. Science, March 24, 2006; 311(5768): 1768 - 1770. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. F. DeLong, C. M. Preston, T. Mincer, V. Rich, S. J. Hallam, N.-U. Frigaard, A. Martinez, M. B. Sullivan, R. Edwards, B. R. Brito, et al. Community Genomics Among Stratified Microbial Assemblages in the Ocean's Interior Science, January 27, 2006; 311(5760): 496 - 503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. R. Zinser, A. Coe, Z. I. Johnson, A. C. Martiny, N. J. Fuller, D. J. Scanlan, and S. W. Chisholm Prochlorococcus Ecotype Abundances in the North Atlantic Ocean As Revealed by an Improved Quantitative PCR Method Appl. Envir. Microbiol., January 1, 2006; 72(1): 723 - 732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Pernthaler and R. Amann Fate of Heterotrophic Microbes in Pelagic Habitats: Focus on Populations Microbiol. Mol. Biol. Rev., September 1, 2005; 69(3): 440 - 461. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. W. Thacker Impacts of Shading on Sponge-Cyanobacteria Symbioses: A Comparison between Host-Specific and Generalist Associations Integr. Comp. Biol., April 1, 2005; 45(2): 369 - 376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Garcia-Fernandez, N. T. de Marsac, and J. Diez Streamlined Regulation and Gene Loss as Adaptive Mechanisms in Prochlorococcus for Optimized Nitrogen Utilization in Oligotrophic Environments Microbiol. Mol. Biol. Rev., December 1, 2004; 68(4): 630 - 638. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Mazard, N. J. Fuller, K. M. Orcutt, O. Bridle, and D. J. Scanlan PCR Analysis of the Distribution of Unicellular Cyanobacterial Diazotrophs in the Arabian Sea Appl. Envir. Microbiol., December 1, 2004; 70(12): 7355 - 7364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Millard, M. R. J. Clokie, D. A. Shub, and N. H. Mann Genetic organization of the psbAD region in phages infecting marine Synechococcus strains PNAS, July 27, 2004; 101(30): 11007 - 11012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Church, H. W. Ducklow, and D. M. Karl Light Dependence of [3H]Leucine Incorporation in the Oligotrophic North Pacific Ocean Appl. Envir. Microbiol., July 1, 2004; 70(7): 4079 - 4087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. C. Biegala, F. Not, D. Vaulot, and N. Simon Quantitative Assessment of Picoeukaryotes in the Natural Environment by Using Taxon-Specific Oligonucleotide Probes in Association with Tyramide Signal Amplification-Fluorescence In Situ Hybridization and Flow Cytometry Appl. Envir. Microbiol., September 1, 2003; 69(9): 5519 - 5529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Fuller, D. Marie, F. Partensky, D. Vaulot, A. F. Post, and D. J. Scanlan Clade-Specific 16S Ribosomal DNA Oligonucleotides Reveal the Predominance of a Single Marine Synechococcus Clade throughout a Stratified Water Column in the Red Sea Appl. Envir. Microbiol., May 1, 2003; 69(5): 2430 - 2443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. V. Zubkov, B. M. Fuchs, G. A. Tarran, P. H. Burkill, and R. Amann High Rate of Uptake of Organic Nitrogen Compounds by Prochlorococcus Cyanobacteria as a Key to Their Dominance in Oligotrophic Oceanic Waters Appl. Envir. Microbiol., February 1, 2003; 69(2): 1299 - 1304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Becker, M. Fahrbach, P. Boger, and A. Ernst Quantitative Tracing, by Taq Nuclease Assays, of a Synechococcus Ecotype in a Highly Diversified Natural Population Appl. Envir. Microbiol., September 1, 2002; 68(9): 4486 - 4494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. O. Casamayor, C. Pedros-Alio, G. Muyzer, and R. Amann Microheterogeneity in 16S Ribosomal DNA-Defined Bacterial Populations from a Stratified Planktonic Environment Is Related to Temporal Changes and to Ecological Adaptations Appl. Envir. Microbiol., April 1, 2002; 68(4): 1706 - 1714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Rocap, D. L. Distel, J. B. Waterbury, and S. W. Chisholm Resolution of Prochlorococcus and Synechococcus Ecotypes by Using 16S-23S Ribosomal DNA Internal Transcribed Spacer Sequences Appl. Envir. Microbiol., March 1, 2002; 68(3): 1180 - 1191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Laloui, K. A. Palinska, R. Rippka, F. Partensky, N. Tandeau de Marsac, M. Herdman, and I. Iteman Genotyping of axenic and non-axenic isolates of the genus Prochlorococcus and the OMF-Synechococcus' clade by size, sequence analysis or RFLP of the Internal Transcribed Spacer of the ribosomal operon Microbiology, February 1, 2002; 148(2): 453 - 465. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
