| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Genetics and Molecular Biology |
Microbiology Department, University College Cork, National University of Ireland, Cork, Ireland1
National Food Biotechnology Centre, National University of Ireland, Cork, Ireland2
Author for correspondence: Alan D. W. Dobson. Tel: +353 21 4902743. Fax: +353 21 4903101. e-mail: a.dobson{at}ucc.ie
Four laccase isozyme genes, Psc lac1, 2, 3 and 4 have been cloned from the edible mushroom, Pleurotus sajor-caju. The genes display a high degree of homology with other basidiomycete laccases (55–99%) at the amino acid level. Of the laccase genes isolated, Psc lac1 and 4 displayed the highest degree of similarity (85% at the amino acid level), while Psc lac3 showed the highest degree of divergence, exhibiting only 52–57% amino acid similarity to the other Pl. sajor-caju laccase gene sequences. Laccase activity in Pl. sajor-caju is affected by nutrient nitrogen and carbon, and by the addition of copper and manganese to the growth medium. In addition, 2,5-xylidine, ferulic acid, veratric acid and 1-hydroxybenzotriazole induced laccase activity in the fungus. Induction of individual laccase isozyme genes by carbon, nitrogen, copper, manganese and the two aromatic compounds, 2,5-xylidine and ferulic acid, occured at the level of gene transcription. While Psc lac3 transcript levels appeared to be constitutively expressed, transcript levels for the other laccase isozyme genes, lac1, 2 and 4, were differentially regulated under the conditions tested.
Keywords: white-rot fungus, transcriptional regulation, ligninolytic enzymes, polyphenol oxidases
Abbreviations: EF, extracellular fluid; FA, ferulic acid; gDNA, genomic DNA; HBT, 1-hydroxybenzotriazole; HC, high carbon; HN, high nitrogen; LNC, low nitrogen and carbon; XYL, 2,5-xylidine
The GenBank accession numbers for the sequences determined in this work are AF297525–AF297528.
This article has been cited by other articles:
|
|
 |
|
  D. C. Canero and M. I. G. Roncero Influence of the chloride channel of Fusarium oxysporum on extracellular laccase activity and virulence on tomato plants Microbiology, May 1, 2008; 154(5): 1474 - 1481. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Canessa, J. M. Alvarez, R. Polanco, P. Bull, and R. Vicuna The copper-dependent ACE1 transcription factor activates the transcription of the mco1 gene from the basidiomycete Phanerochaete chrysosporium Microbiology, February 1, 2008; 154(2): 491 - 499. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Junghanns, M. Moeder, G. Krauss, C. Martin, and D. Schlosser Degradation of the xenoestrogen nonylphenol by aquatic fungi and their laccases Microbiology, January 1, 2005; 151(1): 45 - 57. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. F. Larrondo, B. Gonzalez, D. Cullen, and R. Vicuna Characterization of a multicopper oxidase gene cluster in Phanerochaete chrysosporium and evidence of altered splicing of the mco transcripts Microbiology, August 1, 2004; 150(8): 2775 - 2783. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Z. Xiao, Q. Chen, J. Hang, Y. Y. Shi, Y. Z. Xiao, J. Wu, Y. Z. Hong, and Y. P. Wang Selective induction, purification and characterization of a laccase isozyme from the basidiomycete Trametes sp. AH28-2 Mycologia, January 1, 2004; 96(1): 26 - 35. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Gonzalez, M. C. Terron, E. J. Zapico, A. Tellez, S. Yague, J. M. Carbajo, and A. E. Gonzalez Use of Multiplex Reverse Transcription-PCR To Study the Expression of a Laccase Gene Family in a Basidiomycetous Fungus Appl. Envir. Microbiol., December 1, 2003; 69(12): 7083 - 7090. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. O'Callaghan, M. X. Caddick, and A. D. W. Dobson A polyketide synthase gene required for ochratoxin A biosynthesis in Aspergillus ochraceus Microbiology, December 1, 2003; 149(12): 3485 - 3491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Faraco, P. Giardina, and G. Sannia Metal-responsive elements in Pleurotus ostreatus laccase gene promoters Microbiology, August 1, 2003; 149(8): 2155 - 2162. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. F. Larrondo, M. Avila, L. Salas, D. Cullen, and R. Vicuna Heterologous expression of laccase cDNA from Ceriporiopsis subvermispora yields copper-activated apoprotein and complex isoform patterns Microbiology, May 1, 2003; 149(5): 1177 - 1182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Soden, J. O'Callaghan, and A. D. W. Dobson Molecular cloning of a laccase isozyme gene from Pleurotus sajor-caju and expression in the heterologous Pichia pastoris host Microbiology, December 1, 2002; 148(12): 4003 - 4014. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Galhaup, S. Goller, C. K. Peterbauer, J. Strauss, and D. Haltrich Characterization of the major laccase isoenzyme from Trametes pubescens and regulation of its synthesis by metal ions Microbiology, July 1, 2002; 148(7): 2159 - 2169. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
