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Microbiology 147 (2001), 1929-1936
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Microbiology (2001), 147, 1929-1936.
© 2001 Society for General Microbiology

Pathogenicity and Medical Microbiology

Mosaic structure of Shiga-toxin-2-encoding phages isolated from Escherichia coli O157:H7 indicates frequent gene exchange between lambdoid phage genomes

Birgit K. Johansen1, Yngvild Wasteson1, Per E. Granum1 and Sigrid Brynestad1

Department of Pharmacology, Microbiology and Food Hygiene, The Norwegian School of Veterinary Science, PO Box 8146 Dep., N-0033 Oslo, Norway1

Author for correspondence: Sigrid Brynestad. Tel: +47 22597052. Fax: +47 22964850. e-mail: sigrid.brynestad{at}veths.no

Shiga-toxin-2 (stx2)-encoding bacteriophages were isolated from Norwegian Escherichia coli O157:H7 isolates of cattle and human origin. The phages were characterized by restriction enzyme analysis, hybridization with probes for toxin genes and selected phage DNA such as the P gene, integrase gene and IS1203, and by PCR studies and partial sequencing of selected DNA regions in the integrase to stx2 region of the phages. The stx2-phage-containing bacteria from which inducible phages were detected produced similar amounts of toxin, as shown by a Vero cell assay. The results indicate that the population of stx2-carrying phages is heterogeneous, although the phages from epidemiologically linked E. coli O157:H7 isolates were similar. There appears to have been frequent recombination of stx2 phages with other lambdoid phages.

Keywords: Stx2-encoding phages, STEC, VTEC

Abbreviations: STEC, Shiga-toxin-producing Escherichia coli; Stx, Shiga toxin




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