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Genetics and Molecular Biology |
Department of Molecular Microbiology, B. Rappaport Faculty of Medicine1, and Department of Clinical Microbiology2, Rambam Medical Center, Haifa 31096, Israel
Author for correspondence: Daniel Kornitzer. Tel: +972 4 829 5258. Fax: +972 4 829 5254. e-mail: danielk{at}tx.technion.ac.il
Simple sequence repeats, due to their high variability, are widely used for molecular epidemiology of pathogenic micro-organisms. However, their usefulness is restricted by their high instability and low information content. Here, a locus, CKTNR, in the fungal pathogen Candida krusei is described which displays considerable sequence, as well as length, heterogeneity. Alleles of this locus, which contains a degenerate trinucleotide repeat, appear to be stable. The CKTNR polymorphism could serve as the basis for a molecular typing system of C. krusei. Furthermore, analysis of the CKTNR allele distribution suggested that C. krusei reproduces mainly clonally.
Keywords: molecular typing, fungal VNTR, ploidy level, sexual versus clonal reproduction
Abbreviations: AP-PCR, arbitrarily primed PCR; VNTR, variable numbers of tandem repeats
The GenBank accession numbers for the sequences determined in this work are AF326279–AF326292.
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