| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Genetics and Molecular Biology |
Lehrstuhl für Mikrobiologie, Technische Universität München, Am Hochanger 4, 85350 Freising, Germany1
Department of Microbiology, DTU, Building 301, 2800 Lyngby, Denmark2
Author for correspondence: Leo Eberl. Tel: +49 8161 715446. Fax: +49 8161 715475. e-mail: EBERL{at}mikro.biologie.tu-muenchen.de
Burkholderia cepacia and Pseudomonas aeruginosa often co-exist as mixed biofilms in the lungs of patients suffering from cystic fibrosis (CF). Here, the isolation of random mini-Tn5 insertion mutants of B. cepacia H111 defective in biofilm formation on an abiotic surface is reported. It is demonstrated that one of these mutants no longer produces N-acylhomoserine lactones (AHLs) due to an inactivation of the cepR gene. cepR and the cepI AHL synthase gene together constitute the cep quorum-sensing system of B. cepacia. By using a gene replacement method, two defined mutants, H111-I and H111-R, were constructed in which cepI and cepR, respectively, had been inactivated. These mutants were used to demonstrate that biofilm formation by B. cepacia H111 requires a functional cep quorum-sensing system. A detailed quantitative analysis of the biofilm structures formed by wild-type and mutant strains suggested that the quorum-sensing system is not involved in the regulation of initial cell attachment, but rather controls the maturation of the biofilm. Furthermore, it is shown that B. cepacia is capable of swarming motility, a form of surface translocation utilized by various bacteria to rapidly colonize appropriate substrata. Evidence is provided that swarming motility of B. cepacia is quorum-sensing-regulated, possibly through the control of biosurfactant production. Complementation of the cepR mutant H111-R with different biosurfactants restored swarming motility while biofilm formation was not significantly increased. This result suggests that swarming motility per se is not essential for biofilm formation on abiotic surfaces.
Keywords: cystic fibrosis, N-acylhomoserine lactone, biosurfactant
Abbreviations: AHL, N-acylhomoserine lactone; CF, cystic fibrosis; CLSM, confocal laser scanning microscopy; GFP, green fluorescent protein; C8-HSL, N-octanoylhomoserine lactone; C6-HSL, N-hexanoylhomoserine lactone; 3-oxo-C6-HSL, N-(3-oxohexanoyl)homoserine lactone
This article has been cited by other articles:
|
|
 |
|
  L. De La Fuente, T. J. Burr, and H. C. Hoch Autoaggregation of Xylella fastidiosa Cells Is Influenced by Type I and Type IV Pili Appl. Envir. Microbiol., September 1, 2008; 74(17): 5579 - 5582. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. R. Suarez-Moreno, J. Caballero-Mellado, and V. Venturi The new group of non-pathogenic plant-associated nitrogen-fixing Burkholderia spp. shares a conserved quorum-sensing system, which is tightly regulated by the RsaL repressor Microbiology, July 1, 2008; 154(7): 2048 - 2059. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. De Soyza, A. Silipo, R. Lanzetta, J. R. Govan, and A. Molinaro Review: Chemical and biological features of Burkholderia cepacia complex lipopolysaccharides Innate Immunity, June 1, 2008; 14(3): 127 - 144. [Abstract] [PDF]
|
|
|
|
 |
|
 C. Niu, K. M. Clemmer, R. A. Bonomo, and P. N. Rather Isolation and Characterization of an Autoinducer Synthase from Acinetobacter baumannii J. Bacteriol., May 1, 2008; 190(9): 3386 - 3392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Rambow-Larsen, G. Rajashekara, E. Petersen, and G. Splitter Putative Quorum-Sensing Regulator BlxR of Brucella melitensis Regulates Virulence Factors Including the Type IV Secretion System and Flagella J. Bacteriol., May 1, 2008; 190(9): 3274 - 3282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rieu, S. Weidmann, D. Garmyn, P. Piveteau, and J. Guzzo agr System of Listeria monocytogenes EGD-e: Role in Adherence and Differential Expression Pattern Appl. Envir. Microbiol., October 1, 2007; 73(19): 6125 - 6133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Devescovi, J. Bigirimana, G. Degrassi, L. Cabrio, J. J. LiPuma, J. Kim, I. Hwang, and V. Venturi Involvement of a Quorum-Sensing-Regulated Lipase Secreted by a Clinical Isolate of Burkholderia glumae in Severe Disease Symptoms in Rice Appl. Envir. Microbiol., August 1, 2007; 73(15): 4950 - 4958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Malott and P. A. Sokol Expression of the bviIR and cepIR Quorum-Sensing Systems of Burkholderia vietnamiensis J. Bacteriol., April 15, 2007; 189(8): 3006 - 3016. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Subsin, C. E. Chambers, M. B. Visser, and P. A. Sokol Identification of Genes Regulated by the cepIR Quorum-Sensing System in Burkholderia cenocepacia by High-Throughput Screening of a Random Promoter Library J. Bacteriol., February 1, 2007; 189(3): 968 - 979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hilton, T. Rosche, B. Froelich, B. Smith, and J. Oliver Capsular Polysaccharide Phase Variation in Vibrio vulnificus Appl. Envir. Microbiol., November 1, 2006; 72(11): 6986 - 6993. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Daniels, S. Reynaert, H. Hoekstra, C. Verreth, J. Janssens, K. Braeken, M. Fauvart, S. Beullens, C. Heusdens, I. Lambrichts, et al. Quorum signal molecules as biosurfactants affecting swarming in Rhizobium etli PNAS, October 3, 2006; 103(40): 14965 - 14970. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. S. Alem, M. D. Y. Oteef, T. H. Flowers, and L. J. Douglas Production of Tyrosol by Candida albicans Biofilms and Its Role in Quorum Sensing and Biofilm Development Eukaryot. Cell, October 1, 2006; 5(10): 1770 - 1779. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Kooi, B. Subsin, R. Chen, B. Pohorelic, and P. A. Sokol Burkholderia cenocepacia ZmpB Is a Broad-Specificity Zinc Metalloprotease Involved in Virulence Infect. Immun., July 1, 2006; 74(7): 4083 - 4093. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Pilatz, K. Breitbach, N. Hein, B. Fehlhaber, J. Schulze, B. Brenneke, L. Eberl, and I. Steinmetz Identification of Burkholderia pseudomallei Genes Required for the Intracellular Life Cycle and In Vivo Virulence. Infect. Immun., June 1, 2006; 74(6): 3576 - 3586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Atkinson, C.-Y. Chang, R. E. Sockett, M. Camara, and P. Williams Quorum Sensing in Yersinia enterocolitica Controls Swimming and Swarming Motility J. Bacteriol., February 15, 2006; 188(4): 1451 - 1461. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wopperer, S. T. Cardona, B. Huber, C. A. Jacobi, M. A. Valvano, and L. Eberl A Quorum-Quenching Approach To Investigate the Conservation of Quorum-Sensing-Regulated Functions within the Burkholderia cepacia Complex Appl. Envir. Microbiol., February 1, 2006; 72(2): 1579 - 1587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. W. P Joshua, C. Guthrie-Irons, A. V. Karlyshev, and B. W. Wren Biofilm formation in Campylobacter jejuni Microbiology, February 1, 2006; 152(2): 387 - 396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Kim and M. G. Surette Coordinated Regulation of Two Independent Cell-Cell Signaling Systems and Swarmer Differentiation in Salmonella enterica Serovar Typhimurium J. Bacteriol., January 15, 2006; 188(2): 431 - 440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Riedel, M. Kothe, B. Kramer, W. Saeb, A. Gotschlich, A. Ammendola, and L. Eberl Computer-Aided Design of Agents That Inhibit the cep Quorum-Sensing System of Burkholderia cenocepacia Antimicrob. Agents Chemother., January 1, 2006; 50(1): 318 - 323. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Farah, M. Vera, D. Morin, D. Haras, C. A. Jerez, and N. Guiliani Evidence for a Functional Quorum-Sensing Type AI-1 System in the Extremophilic Bacterium Acidithiobacillus ferrooxidans Appl. Envir. Microbiol., November 1, 2005; 71(11): 7033 - 7040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. C. Caiazza, R. M. Q. Shanks, and G. A. O'Toole Rhamnolipids Modulate Swarming Motility Patterns of Pseudomonas aeruginosa J. Bacteriol., November 1, 2005; 187(21): 7351 - 7361. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Tomlin, R. J. Malott, G. Ramage, D. G. Storey, P. A. Sokol, and H. Ceri Quorum-Sensing Mutations Affect Attachment and Stability of Burkholderia cenocepacia Biofilms Appl. Envir. Microbiol., September 1, 2005; 71(9): 5208 - 5218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Visick and C. Fuqua Decoding Microbial Chatter: Cell-Cell Communication in Bacteria J. Bacteriol., August 15, 2005; 187(16): 5507 - 5519. [Full Text] [PDF]
|
|
|
|
|
|
R. J. Malott, A. Baldwin, E. Mahenthiralingam, and P. A. Sokol Characterization of the cciIR Quorum-Sensing System in Burkholderia cenocepacia Infect. Immun., August 1, 2005; 73(8): 4982 - 4992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Bernier and P. A. Sokol Use of Suppression-Subtractive Hybridization To Identify Genes in the Burkholderia cepacia Complex That Are Unique to Burkholderia cenocepacia J. Bacteriol., August 1, 2005; 187(15): 5278 - 5291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Rice, K. S. Koh, S. Y. Queck, M. Labbate, K. W. Lam, and S. Kjelleberg Biofilm Formation and Sloughing in Serratia marcescens Are Controlled by Quorum Sensing and Nutrient Cues J. Bacteriol., May 15, 2005; 187(10): 3477 - 3485. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. B. Rasmussen, M. E. Skindersoe, T. Bjarnsholt, R. K. Phipps, K. B. Christensen, P. O. Jensen, J. B. Andersen, B. Koch, T. O. Larsen, M. Hentzer, et al. Identity and effects of quorum-sensing inhibitors produced by Penicillium species Microbiology, May 1, 2005; 151(5): 1325 - 1340. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Hoffmann, T. B. Rasmussen, P. Jensen, C. Stub, M. Hentzer, S. Molin, O. Ciofu, M. Givskov, H. K. Johansen, and N. Hoiby Novel Mouse Model of Chronic Pseudomonas aeruginosa Lung Infection Mimicking Cystic Fibrosis Infect. Immun., April 1, 2005; 73(4): 2504 - 2514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Ortega, T. A. Hunt, S. Loutet, A. D. Vinion-Dubiel, A. Datta, B. Choudhury, J. B. Goldberg, R. Carlson, and M. A. Valvano Reconstitution of O-Specific Lipopolysaccharide Expression in Burkholderia cenocepacia Strain J2315, Which Is Associated with Transmissible Infections in Patients with Cystic Fibrosis J. Bacteriol., February 15, 2005; 187(4): 1324 - 1333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-J. Liaw, H.-C. Lai, and W.-B. Wang Modulation of Swarming and Virulence by Fatty Acids through the RsbA Protein in Proteus mirabilis Infect. Immun., December 1, 2004; 72(12): 6836 - 6845. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Huber, F. Feldmann, M. Kothe, P. Vandamme, J. Wopperer, K. Riedel, and L. Eberl Identification of a Novel Virulence Factor in Burkholderia cenocepacia H111 Required for Efficient Slow Killing of Caenorhabditis elegans Infect. Immun., December 1, 2004; 72(12): 7220 - 7230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Sturgill, C. M. Toutain, J. Komperda, G. A. O'Toole, and P. N. Rather Role of CysE in Production of an Extracellular Signaling Molecule in Providencia stuartii and Escherichia coli: Loss of cysE Enhances Biofilm Formation in Escherichia coli J. Bacteriol., November 15, 2004; 186(22): 7610 - 7617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Ulrich, D. DeShazer, H. B. Hines, and J. A. Jeddeloh Quorum Sensing: a Transcriptional Regulatory System Involved in the Pathogenicity of Burkholderia mallei Infect. Immun., November 1, 2004; 72(11): 6589 - 6596. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Ong, C. H. Ooi, D. Wang, H. Chong, K. C. Ng, F. Rodrigues, M. A. Lee, and P. Tan Patterns of large-scale genomic variation in virulent and avirulent Burkholderia species Genome Res., November 1, 2004; 14(11): 2295 - 2307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. C. Petersen, D. Pecharki, and A. A. Scheie Biofilm Mode of Growth of Streptococcus intermedius Favored by a Competence-Stimulating Signaling Peptide J. Bacteriol., September 15, 2004; 186(18): 6327 - 6331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Parsek and C. Fuqua Biofilms 2003: Emerging Themes and Challenges in Studies of Surface-Associated Microbial Life J. Bacteriol., July 15, 2004; 186(14): 4427 - 4440. [Full Text] [PDF]
|
|
|
|
 |
|
 M. V. Cunha, S. A. Sousa, J. H. Leitao, L. M. Moreira, P. A. Videira, and I. Sa-Correia Studies on the Involvement of the Exopolysaccharide Produced by Cystic Fibrosis-Associated Isolates of the Burkholderia cepacia Complex in Biofilm Formation and in Persistence of Respiratory Infections J. Clin. Microbiol., July 1, 2004; 42(7): 3052 - 3058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Ulrich, H. B. Hines, N. Parthasarathy, and J. A. Jeddeloh Mutational Analysis and Biochemical Characterization of the Burkholderia thailandensis DW503 Quorum-Sensing Network J. Bacteriol., July 1, 2004; 186(13): 4350 - 4360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Vallet, S. P. Diggle, R. E. Stacey, M. Camara, I. Ventre, S. Lory, A. Lazdunski, P. Williams, and A. Filloux Biofilm Formation in Pseudomonas aeruginosa: Fimbrial cup Gene Clusters Are Controlled by the Transcriptional Regulator MvaT J. Bacteriol., May 1, 2004; 186(9): 2880 - 2890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Yarwood, D. J. Bartels, E. M. Volper, and E. P. Greenberg Quorum Sensing in Staphylococcus aureus Biofilms J. Bacteriol., March 15, 2004; 186(6): 1838 - 1850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Labbate, S. Y. Queck, K. S. Koh, S. A. Rice, M. Givskov, and S. Kjelleberg Quorum Sensing-Controlled Biofilm Development in Serratia liquefaciens MG1 J. Bacteriol., February 1, 2004; 186(3): 692 - 698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. G. MacLehose, P. Gilbert, and D. G. Allison Biofilms, homoserine lactones and biocide susceptibility J. Antimicrob. Chemother., February 1, 2004; 53(2): 180 - 184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Sokol, U. Sajjan, M. B. Visser, S. Gingues, J. Forstner, and C. Kooi The CepIR quorum-sensing system contributes to the virulence of Burkholderia cenocepacia respiratory infections Microbiology, December 1, 2003; 149(12): 3649 - 3658. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Aguilar, A. Friscina, G. Devescovi, M. Kojic, and V. Venturi Identification of Quorum-Sensing-Regulated Genes of Burkholderia cepacia J. Bacteriol., November 1, 2003; 185(21): 6456 - 6462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Dow, L. Crossman, K. Findlay, Y.-Q. He, J.-X. Feng, and J.-L. Tang Biofilm dispersal in Xanthomonas campestris is controlled by cell-cell signaling and is required for full virulence to plants PNAS, September 16, 2003; 100(19): 10995 - 11000. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Webb, L. S. Thompson, S. James, T. Charlton, T. Tolker-Nielsen, B. Koch, M. Givskov, and S. Kjelleberg Cell Death in Pseudomonas aeruginosa Biofilm Development J. Bacteriol., August 1, 2003; 185(15): 4585 - 4592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Merritt, F. Qi, S. D. Goodman, M. H. Anderson, and W. Shi Mutation of luxS Affects Biofilm Formation in Streptococcus mutans Infect. Immun., April 1, 2003; 71(4): 1972 - 1979. [Abstract] [Full Text]
|
|
|
|
|
|
C. Aguilar, I. Bertani, and V. Venturi Quorum-Sensing System and Stationary-Phase Sigma Factor (rpoS) of the Onion Pathogen Burkholderia cepacia Genomovar I Type Strain, ATCC 25416 Appl. Envir. Microbiol., March 1, 2003; 69(3): 1739 - 1747. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Chung, E. Altman, T. J. Beveridge, and D. P. Speert Colonial Morphology of Burkholderia cepacia Complex Genomovar III: Implications in Exopolysaccharide Production, Pilus Expression, and Persistence in the Mouse Infect. Immun., February 1, 2003; 71(2): 904 - 909. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. Christensen, K. Riedel, L. Eberl, L. R. Flodgaard, S. Molin, L. Gram, and M. Givskov Quorum-sensing-directed protein expression in Serratia proteamaculans B5a Microbiology, February 1, 2003; 149(2): 471 - 483. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Andersen, B. Koch, T. H. Nielsen, D. Sorensen, M. Hansen, O. Nybroe, C. Christophersen, J. Sorensen, S. Molin, and M. Givskov Surface motility in Pseudomonas sp. DSS73 is required for efficient biological containment of the root-pathogenic microfungi Rhizoctonia solani and Pythium ultimum Microbiology, January 1, 2003; 149(1): 37 - 46. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Steidle, M. Allesen-Holm, K. Riedel, G. Berg, M. Givskov, S. Molin, and L. Eberl Identification and Characterization of an N-Acylhomoserine Lactone-Dependent Quorum-Sensing System in Pseudomonas putida Strain IsoF Appl. Envir. Microbiol., December 1, 2002; 68(12): 6371 - 6382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-A. D. Conway, V. Venu, and D. P. Speert Biofilm Formation and Acyl Homoserine Lactone Production in the Burkholderia cepacia Complex J. Bacteriol., October 15, 2002; 184(20): 5678 - 5685. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Schwab, M. Leigh, C. Ribeiro, J. Yankaskas, K. Burns, P. Gilligan, P. Sokol, and R. Boucher Patterns of Epithelial Cell Invasion by Different Species of the Burkholderia cepacia Complex in Well-Differentiated Human Airway Epithelia Infect. Immun., August 1, 2002; 70(8): 4547 - 4555. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Schembri, M. Givskov, and P. Klemm An Attractive Surface: Gram-Negative Bacterial Biofilms Sci. Signal., May 14, 2002; 2002(132): re6 - re6. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Riedel, M. Hentzer, O. Geisenberger, B. Huber, A. Steidle, H. Wu, N. Hoiby, M. Givskov, S. Molin, and L. Eberl N-Acylhomoserine-lactone-mediated communication between Pseudomonas aeruginosa and Burkholderia cepacia in mixed biofilms Microbiology, December 1, 2001; 147(12): 3249 - 3262. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
