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Research Paper |
Department of Medicine, Division of Hematology–Oncology, University of Pennsylvania, Philadelphia, PA 19104, USA1
Laboratoire d’Ingénierie des Protéines et Contrôle Métabolique, Institut Jacques Monod, Tour 43, Université Paris 7/Paris 6, 2 Place Jussieu, 75251 Paris Cedex 05, France2
Office of the Director, NCI, National Institutes of Health, Bethesda, MD 20892, USA3
Author for correspondence: Andrew Dancis. Tel: +1 215 573 6275. Fax: +1 215 573 7049. e-mail: adancis{at}mail.med.upenn.edu
High-affinity iron uptake by a ferrous permease in the opportunistic pathogen Candida albicans is required for virulence. Here this iron uptake system has been characterized by investigating three distinct activities: an externally directed surface ferric reductase, a membrane-associated PPD (p-phenylenediamine) oxidase and a cellular ferrous iron transport activity. Copper was required for the PPD oxidase and ferrous transport activities. In contrast, copper was not required for iron uptake from siderophores. Addition of iron to the growth medium repressed ferric reductase and ferrous transport, indicating homeostatic regulation. To identify the genes involved, orthologous mutants of Saccharomyces cerevisiae were transformed with a genomic library of C. albicans. CFL95, a gene with sequence similarity to ferric reductases, restored reductase activity to the orthologous S. cerevisiae mutant. CaFTR2 and CaFTR1, genes with homology to ferrous permeases, conferred ferrous transport activity to the orthologous S. cerevisiae mutant. However, neither a genomic library nor CaFET99, a multicopper oxidase homologue and candidate gene for the PPD oxidase, complemented the S. cerevisiae mutant, possibly because of problems with targeting or assembly. Transcripts for CFL95, CaFTR1 and CaFET99 were strongly repressed by iron, whereas the CaFTR2 transcript was induced by iron. Deletion of the TUP1 regulator perturbed the homeostatic control of reductive iron uptake. Incidentally, iron starvation was noted to induce flavin production and this was misregulated in the absence of TUP1 control. The opposite regulation of two iron permease genes and the role of TUP1 indicate that the process of iron acquisition by C. albicans may be more complex and potentially more adaptable than by S. cerevisiae.
Keywords: iron regulation, ferric reductase, multicopper oxidase, ferrous permease, siderophores
Abbreviations: BCS, bathocuproinedisulfonate; BPS, bathophenanthrolinedisulfonate; CSM, complete synthetic medium; PGK, phosphoglyerol kinase; PPD, p-phenylenediamine
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