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Molecular Genetics and Immunobiology of Mycobacteria |
Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex, France1
Annotation-Bases de Données (PT4), Génopole, Institut Pasteur, Paris, France2
Génoscope/UMR 8030, Atelier de Génomique Comparative, 2 rue Gaston Crémieux, 91006 Evry Cedex, France3
Author for correspondence: Stewart T. Cole. Tel: +33 1 45688446. Fax: +33 1 40613583. e-mail: stcole{at}pasteur.fr
Original genome annotations need to be regularly updated if the information they contain is to remain accurate and relevant. Here the complete re-annotation of the genome sequence of Mycobacterium tuberculosis strain H37Rv is presented almost 4 years after the first submission. Eighty-two new protein-coding sequences (CDS) have been included and 22 of these have a predicted function. The majority were identified by manual or automated re-analysis of the genome and most of them were shorter than the 100 codon cut-off used in the initial genome analysis. The functional classification of 643 CDS has been changed based principally on recent sequence comparisons and new experimental data from the literature. More than 300 gene names and over 1000 targeted citations have been added and the lengths of 60 genes have been modified. Presently, it is possible to assign a function to 2058 proteins (52% of the 3995 proteins predicted) and only 376 putative proteins share no homology with known proteins and thus could be unique to M. tuberculosis.
Keywords: mycobacteria, tuberculosis, genomics
Abbreviations: CDS, protein-coding sequences
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