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Microbiology 148 (2002), 3039-3048
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Microbiology (2002), 148, 3039-3048.
© 2002 Society for General Microbiology

Molecular Genetics and Immunobiology of Mycobacteria

Synthesis of an unusual polar glycopeptidolipid in glucose-limited culture of Mycobacterium smegmatis

Anil Kr. Ojha1,2, Saaket Varma1 and Dipankar Chatterji1

Molecular Biophysics Unit, Indian Institute of Science, Bangalore, 560012, India1
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad. 500007, India2

Author for correspondence: Dipankar Chatterji. Tel: +91 80 309 2836. Fax: +91 80 360 0535. e-mail: dipankar{at}mbu.iisc.ernet.in

There has been a general understanding that Mycobacterium smegmatis produces only apolar glycopeptidolipid (GPL), similar in structure to serovar non-specific GPL of Mycobacterium avium. In this study, synthesis of polar GPL in carbon-starved M. smegmatis is reported. Mass spectrometric analysis suggests the polar GPL to be a hyperglycosylated species. The earlier structural studies of polar GPLs from M. avium have invariably shown the presence of an oligosaccharide appendage to D-allo-Thr. However, a further chemical analysis using ß-elimination of the newly found polar GPL in M. smegmatis shows that the molecule still contains a monosaccharide at the D-allo-Thr, thus suggesting a new form of polar GPL.

Keywords: polar glycopeptidolipid, carbon starvation

Abbreviations: 6-dTal, 6-deoxy talose; ESI-MS, electrospray ionization mass spectrometry; GPL, glycopeptidolipid; MALDI-TOF, matrix assisted laser desorption ionization-time of flight; nsGPL, non-specific GPL




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