| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Research Paper |
Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA1
Department of Medical Microbiology, University of Groningen, PO Box 30001, 9700 RB Groningen, The Netherlands2
Department of Cell Biology, Histology and Immunology Section, University of Groningen, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands3
Author for correspondence: Nita H. Salzman. Tel: +1 414 456 4244. Fax: +1 414 456 6535. e-mail: nsalzman{at}mcw.edu
Total genomic DNA from samples of intact mouse small intestine, large intestine, caecum and faeces was used as template for PCR amplification of 16S rRNA gene sequences with conserved bacterial primers. Phylogenetic analysis of the amplification products revealed 40 unique 16S rDNA sequences. Of these sequences, 25% (10/40) corresponded to described intestinal organisms of the mouse, including Lactobacillus spp., Helicobacter spp., segmented filamentous bacteria and members of the altered Schaedler flora (ASF360, ASF361, ASF502 and ASF519); 75% (30/40) represented novel sequences. A large number (11/40) of the novel sequences revealed a new operational taxonomic unit (OTU) belonging to the Cytophaga–Flavobacter–Bacteroides phylum, which the authors named ‘mouse intestinal bacteria’. 16S rRNA probes were developed for this new OTU. Upon analysis of the novel sequences, eight were found to cluster within the Eubacterium rectale–Clostridium coccoides group and three clustered within the Bacteroides group. One of the novel sequences was distantly related to Verrucomicrobium spinosum and one was distantly related to Bacillus mycoides. Oligonucleotide probes specific for the 16S rRNA of these novel clones were generated. Using a combination of four previously described and four newly designed probes, approximately 80% of bacteria recovered from the murine large intestine and 71% of bacteria recovered from the murine caecum could be identified by fluorescence in situ hybridization (FISH).
|
Abbreviations: DAPI, 4',6-diamidino-2-phenylindole; FISH, fluorescence in situ hybridization; MIB, ‘mouse intestinal bacteria’; OTU, operational taxonomic unit; SFB, segmented filamentous bacteria
b The GenBank accession numbers for the clone sequences reported in this paper can be found in Table 1; the accession number for isolate MIB-CB3 is AJ418059.
a Present address: Medical College of Wisconsin, Department of Pediatrics, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA.
This article has been cited by other articles:
|
|
 |
|
  M. Hasegawa, T. Osaka, K. Tawaratsumida, T. Yamazaki, H. Tada, G. Y. Chen, S. Tsuneda, G. Nunez, and N. Inohara Transitions in Oral and Intestinal Microflora Composition and Innate Immune Receptor-Dependent Stimulation during Mouse Development Infect. Immun., February 1, 2010; 78(2): 639 - 650. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Stehr, M. C Greweling, S. Tischer, M. Singh, H. Blocker, D. A Monner, and W. Muller Charles River altered Schaedler flora (CRASF(R)) remained stable for four years in a mouse colony housed in individually ventilated cages Lab Anim, October 1, 2009; 43(4): 362 - 370. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Croswell, E. Amir, P. Teggatz, M. Barman, and N. H. Salzman Prolonged Impact of Antibiotics on Intestinal Microbial Ecology and Susceptibility to Enteric Salmonella Infection Infect. Immun., July 1, 2009; 77(7): 2741 - 2753. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. D. Garner, D. A. Antonopoulos, B. Wagner, G. E. Duhamel, I. Keresztes, D. A. Ross, V. B. Young, and C. Altier Perturbation of the Small Intestine Microbial Ecology by Streptomycin Alters Pathology in a Salmonella enterica Serovar Typhimurium Murine Model of Infection Infect. Immun., July 1, 2009; 77(7): 2691 - 2702. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Clavel, C. Charrier, A. Braune, M. Wenning, M. Blaut, and D. Haller Isolation of bacteria from the ileal mucosa of TNFdeltaARE mice and description of Enterorhabdus mucosicola gen. nov., sp. nov. Int J Syst Evol Microbiol, July 1, 2009; 59(7): 1805 - 1812. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Walter Ecological Role of Lactobacilli in the Gastrointestinal Tract: Implications for Fundamental and Biomedical Research Appl. Envir. Microbiol., August 15, 2008; 74(16): 4985 - 4996. [Full Text] [PDF]
|
|
|
|
|
|
M. Barman, D. Unold, K. Shifley, E. Amir, K. Hung, N. Bos, and N. Salzman Enteric Salmonellosis Disrupts the Microbial Ecology of the Murine Gastrointestinal Tract Infect. Immun., March 1, 2008; 76(3): 907 - 915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Derrien, M. C. Collado, K. Ben-Amor, S. Salminen, and W. M. de Vos The Mucin Degrader Akkermansia muciniphila Is an Abundant Resident of the Human Intestinal Tract Appl. Envir. Microbiol., March 1, 2008; 74(5): 1646 - 1648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Voltan, I. Castagliuolo, M. Elli, S. Longo, P. Brun, R. D'Inca, A. Porzionato, V. Macchi, G. Palu, G. C. Sturniolo, et al. Aggregating Phenotype in Lactobacillus crispatus Determines Intestinal Colonization and TLR2 and TLR4 Modulation in Murine Colonic Mucosa Clin. Vaccine Immunol., September 1, 2007; 14(9): 1138 - 1148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. D. Cani, J. Amar, M. A. Iglesias, M. Poggi, C. Knauf, D. Bastelica, A. M. Neyrinck, F. Fava, K. M. Tuohy, C. Chabo, et al. Metabolic Endotoxemia Initiates Obesity and Insulin Resistance Diabetes, July 1, 2007; 56(7): 1761 - 1772. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Nakanishi, K. Murashima, H. Ohara, T. Suzuki, H. Hayashi, M. Sakamoto, T. Fukasawa, H. Kubota, A. Hosono, T. Kono, et al. Increase in Terminal Restriction Fragments of Bacteroidetes-Derived 16S rRNA Genes after Administration of Short-Chain Fructooligosaccharides Appl. Envir. Microbiol., September 1, 2006; 72(9): 6271 - 6276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Ge, Y. Feng, N. S. Taylor, M. Ohtani, M. F. Polz, D. B. Schauer, and J. G. Fox Colonization Dynamics of Altered Schaedler Flora Is Influenced by Gender, Aging, and Helicobacter hepaticus Infection in the Intestines of Swiss Webster Mice. Appl. Envir. Microbiol., July 1, 2006; 72(7): 5100 - 5103. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Lucke, S. Miehlke, E. Jacobs, and M. Schuppler Prevalence of Bacteroides and Prevotella spp. in ulcerative colitis. J. Med. Microbiol., May 1, 2006; 55(Pt 5): 617 - 624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Dinoto, A. Suksomcheep, S. Ishizuka, H. Kimura, S. Hanada, Y. Kamagata, K. Asano, F. Tomita, and A. Yokota Modulation of Rat Cecal Microbiota by Administration of Raffinose and Encapsulated Bifidobacterium breve Appl. Envir. Microbiol., January 1, 2006; 72(1): 784 - 792. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J Scupham, L. L. Presley, B. Wei, E. Bent, N. Griffith, M. McPherson, F. Zhu, O. Oluwadara, N. Rao, J. Braun, et al. Abundant and Diverse Fungal Microbiota in the Murine Intestine Appl. Envir. Microbiol., January 1, 2006; 72(1): 793 - 801. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wehkamp, N. H. Salzman, E. Porter, S. Nuding, M. Weichenthal, R. E. Petras, B. Shen, E. Schaeffeler, M. Schwab, R. Linzmeier, et al. Reduced Paneth cell {alpha}-defensins in ileal Crohn's disease PNAS, December 13, 2005; 102(50): 18129 - 18134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Kuehl, H. D. Wood, T. L. Marsh, T. M. Schmidt, and V. B. Young Colonization of the Cecal Mucosa by Helicobacter hepaticus Impacts the Diversity of the Indigenous Microbiota Infect. Immun., October 1, 2005; 73(10): 6952 - 6961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kibe, M. Sakamoto, H. Yokota, H. Ishikawa, Y. Aiba, Y. Koga, and Y. Benno Movement and Fixation of Intestinal Microbiota after Administration of Human Feces to Germfree Mice Appl. Envir. Microbiol., June 1, 2005; 71(6): 3171 - 3178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Walter, M. Mangold, and G. W. Tannock Construction, Analysis, and {beta}-Glucanase Screening of a Bacterial Artificial Chromosome Library from the Large-Bowel Microbiota of Mice Appl. Envir. Microbiol., May 1, 2005; 71(5): 2347 - 2354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Hill, S. M. Hemmingsen, B. G. Goldade, T. J. Dumonceaux, J. Klassen, R. T. Zijlstra, S. H. Goh, and A. G. Van Kessel Comparison of Ileum Microflora of Pigs Fed Corn-, Wheat-, or Barley-Based Diets by Chaperonin-60 Sequencing and Quantitative PCR Appl. Envir. Microbiol., February 1, 2005; 71(2): 867 - 875. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Song, C. Liu, and S. M. Finegold Real-Time PCR Quantitation of Clostridia in Feces of Autistic Children Appl. Envir. Microbiol., November 1, 2004; 70(11): 6459 - 6465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Derrien, E. E. Vaughan, C. M. Plugge, and W. M. de Vos Akkermansia muciniphila gen. nov., sp. nov., a human intestinal mucin-degrading bacterium Int J Syst Evol Microbiol, September 1, 2004; 54(5): 1469 - 1476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. B. Sarma-Rupavtarm, Z. Ge, D. B. Schauer, J. G. Fox, and M. F. Polz Spatial Distribution and Stability of the Eight Microbial Species of the Altered Schaedler Flora in the Mouse Gastrointestinal Tract Appl. Envir. Microbiol., May 1, 2004; 70(5): 2791 - 2800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Schuppler, K. Lotzsch, M. Waidmann, and I. B. Autenrieth An Abundance of Escherichia coli Is Harbored by the Mucosa- Associated Bacterial Flora of Interleukin-2-Deficient Mice Infect. Immun., April 1, 2004; 72(4): 1983 - 1990. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Rawls, B. S. Samuel, and J. I. Gordon From The Cover: Gnotobiotic zebrafish reveal evolutionarily conserved responses to the gut microbiota PNAS, March 30, 2004; 101(13): 4596 - 4601. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
