Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 148 (2002), 3681-3693
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jermyn, W. S.
Right arrow Articles by Boyd, E. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jermyn, W. S.
Right arrow Articles by Boyd, E. F.
Agricola
Right arrow Articles by Jermyn, W. S.
Right arrow Articles by Boyd, E. F.
Microbiology (2002), 148, 3681-3693.
© 2002 Society for General Microbiology

Research Paper

Characterization of a novel Vibrio pathogenicity island (VPI-2) encoding neuraminidase (nanH) among toxigenic Vibrio cholerae isolates

William S. Jermyn1 and E. Fidelma Boyd1

Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland1

Author for correspondence: E. Fidelma Boyd. Tel: +353 21 4903624. Fax: +353 21 4903101. e-mail: f.boyd{at}ucc.ie

Acquisition of virulence genes encoded on mobile genetic elements has played an important role in the emergence of pathogenic isolates of Vibrio cholerae, the causative agent of the diarrhoeal disease cholera. The genes encoding cholera toxin (ctxAB), the main cause of profuse secretory diarrhoea in cholera, are encoded on a filamentous bacteriophage CTX{phi}. The toxin coregulated pilus (TCP), an essential intestinal colonization factor, was originally designated as part of a pathogenicity island named the Vibrio pathogenicity island (VPI), but this island has more recently been proposed to be the genome of a filamentous phage, VPI{phi}. In this study, it is shown that nanH, which encodes neuraminidase, maps within a novel pathogenicity island designated VPI-2. The 57·3 kb VPI-2 has all of the characteristic features of a pathogenicity island, including the presence of a bacteriophage-like integrase (int), insertion in a tRNA gene (serine) and the presence of direct repeats at the chromosomal integration sites. Additionally, the G+C content of VPI-2 (42 mol%) is considerably lower than that of the entire genome (47 mol%). VPI-2 encodes several gene clusters, such as a restriction modification system (hsdR and hsdM) and genes required for the utilization of amino sugars (nan-nag region) as well as neuraminidase. To determine the distribution of VPI-2 among V. cholerae, 78 natural isolates were examined using PCR and Southern hybridization analysis for the presence of this region. All toxigenic V. cholerae O1 serogroup isolates examined contained VPI-2, whereas non-toxigenic isolates lacked the island. Of 14 V. cholerae O139 serogroup isolates examined, only one strain, MO2, contained the entire 57·3 kb island, whereas 13 O139 isolates contained only a 20·0 kb region with most of the 5' region of VPI-2 which included nanH deleted in these strains.

Keywords: virulence factors, bacteriophage, restriction modification

Abbreviations: CT, cholera toxin; IS, insertion sequence; TCP, toxin coregulated pilus; Vibrio pathogenicity island




This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
R. A. Murphy and E. F. Boyd
Three Pathogenicity Islands of Vibrio cholerae Can Excise from the Chromosome and Form Circular Intermediates
J. Bacteriol., January 15, 2008; 190(2): 636 - 647.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M.-J. Lombardo, J. Michalski, H. Martinez-Wilson, C. Morin, T. Hilton, C. G. Osorio, J. P. Nataro, C. O. Tacket, A. Camilli, and J. B. Kaper
An in vivo expression technology screen for Vibrio cholerae genes expressed in human volunteers
PNAS, November 13, 2007; 104(46): 18229 - 18234.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
B. Pang, M. Yan, Z. Cui, X. Ye, B. Diao, Y. Ren, S. Gao, L. Zhang, and B. Kan
Genetic Diversity of Toxigenic and Nontoxigenic Vibrio cholerae Serogroups O1 and O139 Revealed by Array-Based Comparative Genomic Hybridization
J. Bacteriol., July 1, 2007; 189(13): 4837 - 4849.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
M. C. Miller, D. P. Keymer, A. Avelar, A. B. Boehm, and G. K. Schoolnik
Detection and Transformation of Genome Segments That Differ within a Coastal Population of Vibrio cholerae Strains
Appl. Envir. Microbiol., June 1, 2007; 73(11): 3695 - 3704.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
Y. Chen, J. A. Johnson, G. D. Pusch, J. G. Morris Jr., and O. C. Stine
The Genome of Non-O1 Vibrio cholerae NRT36S Demonstrates the Presence of Pathogenic Mechanisms That Are Distinct from Those of O1 Vibrio cholerae
Infect. Immun., May 1, 2007; 75(5): 2645 - 2647.
[Abstract] [Full Text] [PDF]

Home page
 BioinformaticsHome page
A. M. Quirke, F. J. Reen, M. J. Claesson, and E. F. Boyd
Genomic island identification in Vibrio vulnificus reveals significant genome plasticity in this human pathogen
Bioinformatics, April 15, 2006; 22(8): 905 - 910.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. J. Vora, C. E. Meador, M. M. Bird, C. A. Bopp, J. D. Andreadis, and D. A. Stenger
Microarray-based detection of genetic heterogeneity, antimicrobial resistance, and the viable but nonculturable state in human pathogenic Vibrio spp.
PNAS, December 27, 2005; 102(52): 19109 - 19114.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Dziejman, D. Serruto, V. C. Tam, D. Sturtevant, P. Diraphat, S. M. Faruque, M. H. Rahman, J. F. Heidelberg, J. Decker, L. Li, et al.
Genomic characterization of non-O1, non-O139 Vibrio cholerae reveals genes for a type III secretion system
PNAS, March 1, 2005; 102(9): 3465 - 3470.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
W. S. Jermyn and E. F. Boyd
Molecular evolution of Vibrio pathogenicity island-2 (VPI-2): mosaic structure among Vibrio cholerae and Vibrio mimicus natural isolates
Microbiology, January 1, 2005; 151(1): 311 - 322.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
Y. A. O'Shea, S. Finnan, F. J. Reen, J. P. Morrissey, F. O'Gara, and E. F. Boyd
The Vibrio seventh pandemic island-II is a 26{middle dot}9 kb genomic island present in Vibrio cholerae El Tor and O139 serogroup isolates that shows homology to a 43{middle dot}4 kb genomic island in V. vulnificus
Microbiology, December 1, 2004; 150(12): 4053 - 4063.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
Y. A. O'Shea, F. J. Reen, A. M. Quirke, and E. F. Boyd
Evolutionary Genetic Analysis of the Emergence of Epidemic Vibrio cholerae Isolates on the Basis of Comparative Nucleotide Sequence Analysis and Multilocus Virulence Gene Profiles
J. Clin. Microbiol., October 1, 2004; 42(10): 4657 - 4671.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Moustafa, H. Connaris, M. Taylor, V. Zaitsev, J. C. Wilson, M. J. Kiefel, M. von Itzstein, and G. Taylor
Sialic Acid Recognition by Vibrio cholerae Neuraminidase
J. Biol. Chem., September 24, 2004; 279(39): 40819 - 40826.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
H. Brussow, C. Canchaya, and W.-D. Hardt
Phages and the Evolution of Bacterial Pathogens: from Genomic Rearrangements to Lysogenic Conversion
Microbiol. Mol. Biol. Rev., September 1, 2004; 68(3): 560 - 602.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
H. Schmidt and M. Hensel
Pathogenicity Islands in Bacterial Pathogenesis
Clin. Microbiol. Rev., January 1, 2004; 17(1): 14 - 56.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2002 Society for General Microbiology.