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Microbiology 148 (2002), 373-379
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Microbiology (2002), 148, 373-379.
© 2002 Society for General Microbiology

Research Paper

Analysis of a C-methyltransferase gene (aviG1) involved in avilamycin biosynthesis in Streptomyces viridochromogenes Tü57 and complementation of a Saccharopolyspora erythraea eryBIII mutant by aviG1

G. Weitnauer1, S. Gaisser2, L. Kellenberger2, P. F. Leadlay2 and A. Bechthold1

Albert-Ludwigs-Universität Freiburg, Institut für Pharmazeutische Biologie, Stefan-Meier Str. 19, 79104 Freiburg, Germany1
Cambridge Centre for Molecular Recognition and Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK2

Author for correspondence: A. Bechthold. Tel: +49 761 2038371. Fax: +49 761 8383. e-mail: andreas.bechthold{at}uni-freiburg.de

Streptomyces viridochromogenes Tü57 is the principal producer of avilamycin A. aviG1, a putative methyltransferase gene, was detected in the avilamycin biosynthetic gene cluster. To determine the function of aviG1, a targeted gene inactivation experiment was performed. The resulting chromosomal mutant, carrying an in-frame deletion in aviG1, was deficient in avilamycin production. aviG1 was used to complement an eryBIII mutant of the erythromycin A producer Saccharopolyspora erythraea [Gaisser, S., Bohm, G. A., Doumith, M., Raynal, M. C., Dhillon, N., Cortes, J. & Leadlay, P. F. (1998) . Mol Gen Genet 258, 78–88]. The presence of erythromycin A in the culture supernatant of the complemented mutant indicated that L-mycarose biosynthesis could be restored and that AviG1 could take over the function of the C-methyltransferase EryBIII.

Keywords: avilamycin A, erythromycin, deoxysugar biosynthetic genes, methyltransferase

Abbreviations: ES-MS, electrospray mass spectrometry

The GenBank accession number for the sequence reported in this paper is AF333038.




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