| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Research Paper |
Unité Flore Lactique et Environnement Carné, INRA, Domaine de Vilvert, F-78350 Jouy-en-Josas, France1
Author for correspondence: Monique Zagorec. Tel: +33 1 34 65 22 89. Fax: +33 1 34 65 21 05. e-mail: zagorec{at}diamant.jouy.inra.fr
The Lactobacillus sakei 23K chromosome was analysed by pulsed-field gel electrophoresis after digestion with the restriction enzymes AscI, NotI and SfiI. The chromosome size was estimated to be 1845±80 kb. The use of I-CeuI, specific for rrn genes encoding 23S rRNAs, showed that seven rrn loci were present, on 40% of the chromosome. The seven rrn clusters were mapped and their orientation was determined, allowing the position of the replication origin to be estimated. Partial I-CeuI digestions were used to construct a backbone and the different restriction fragments obtained with AscI, NotI and SfiI were assembled to a physical map by Southern hybridization. Eleven L. sakei gene clusters previously identified were mapped, as well as 25 new loci located randomly on the chromosome and 11 regions flanking the rrn gene clusters. A total of 47 clusters were thus mapped on L. sakei chromosome. The new loci were sequenced, allowing the identification of 73 complete or incomplete coding sequences. Among these 73 new genes of L. sakei, the function of 36 could be deduced from their similarity to known genes described in databases. However, 10 genes had no homologues, 10 encoded proteins similar to proteins of unknown function and 17 were similar to hypothetical proteins.
Keywords: lactic acid bacteria, meat, PFGE
Abbreviations: LM-PCR, ligation-mediated polymerase chain reaction
The GenBank accession numbers for the sequences reported in this paper are given in Table 2 and the legend to Fig. 3.
a Present address: Institute of Food Research, Norwich Research Park, Norwich NR4 7UA, UK.
b Present address: Unité Ecologie et Physiologie du Système Digestif, INRA, Domaine de Vilvert, F78350 Jouy-en-Josas, France.
This article has been cited by other articles:
|
|
 |
|
  E. Sorvig, G. Mathiesen, K. Naterstad, V. G. H. Eijsink, and L. Axelsson High-level, inducible gene expression in Lactobacillus sakei and Lactobacillus plantarum using versatile expression vectors Microbiology, July 1, 2005; 151(7): 2439 - 2449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. G. A. EL-Osta, A. J. Hillier, and M. Dobos Construction of a combined physical and genetic map of the chromosome of Lactobacillus acidophilus ATCC 4356 and characterization of the rRNA operons Microbiology, March 1, 2005; 151(3): 875 - 892. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Marceau, M. Zagorec, S. Chaillou, T. Mera, and M.-C. Champomier-Verges Evidence for Involvement of at Least Six Proteins in Adaptation of Lactobacillus sakei to Cold Temperatures and Addition of NaCl Appl. Envir. Microbiol., December 1, 2004; 70(12): 7260 - 7268. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. M. Chelo, L. Ze-Ze, L. Chambel, and R. Tenreiro Physical and genetic map of the Weissella paramesenteroides DSMZ 20288T chromosome and characterization of different rrn operons by ITS analysis Microbiology, December 1, 2004; 150(12): 4075 - 4084. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-C. Champomier-Verges, A. Marceau, T. Mera, and M. Zagorec The pepR Gene of Lactobacillus sakei Is Positively Regulated by Anaerobiosis at the Transcriptional Level Appl. Envir. Microbiol., August 1, 2002; 68(8): 3873 - 3877. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
