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Microbiology 148 (2002), 443-451
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Microbiology (2002), 148, 443-451.
© 2002 Society for General Microbiology

Research Paper

Phage-display antibody detection of Chlamydia trachomatis-associated antigens

Erika A. Lindquist1, James D. Marks2, Betsy J. Kleba1 and Richard S. Stephens1,3

Division of Infectious Diseases, School of Public Health, 235 Earl Warren Hall, University of California, Berkeley, CA 94720-7360, USA1
Departments of Anesthesia and Pharmaceutical Chemistry2 and the Francis I. Proctor Foundation3, University of California, San Francisco, CA 94143, USA

Author for correspondence: Richard S. Stephens. Tel: +1 510 643 9900. Fax: +1 510 643 1537. e-mail: rss{at}uclink4.berkeley.edu

A phage-displayed human single-chain Fv antibody library (6·7x109 members) was used to select probes specific to components associated with the surface of Chlamydia trachomatis elementary bodies (EBs). Each of 15 antibodies was characterized by ELISA, dot-blot, immunoblot and immunocytochemistry, resulting in the identification of several new chlamydial components associated with the surface of EBs. In addition, six antibodies were specific for host-cell components associated with the surface of EBs. While phage display has been used effectively to produce specific antibodies for purified components, these data show that this technology is suitable for selection of specific probes from complex antigens such as the surface of a microbial pathogen.

Keywords: intracellular bacteria, outer-membrane proteins, recombinant antibody

Abbreviations: EB, elementary body; HRP, horesradish peroxidase; scFv, single-chain variable fragment




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B. J. Kleba, E. Banta, E. A. Lindquist, and R. S. Stephens
Recruitment of Mammalian Cell Fibronectin to the Surface of Chlamydia trachomatis
Infect. Immun., July 1, 2002; 70(7): 3935 - 3938.
[Abstract] [Full Text] [PDF]


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