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Microbiology 148 (2002), 1183-1191
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Microbiology (2002), 148, 1183-1191.
© 2002 Society for General Microbiology

Research Paper

Construction and characterization of a nonpigmented mutant of Porphyromonas gingivalis: cell surface polysaccharide as an anchorage for gingipains

Mikio Shojia,b,1,2, Dinath B. Ratnayakea,1, Yixin Shi1, Tomoko Kadowaki3, Kenji Yamamoto3, Fuminobu Yoshimura4, Akifumi Akamine2, Michael A. Curtis5 and Koji Nakayama6

Departments of Oral Infectious Diseases and Immunology1, Endodontology and Operative Dentistry2 and Pharmacology3, Faculty of Dental Science, Kyushu University, Fukuoka812-8582, Japan
Department of Microbiology, School of Dentistry, Aichi-Gakuin University, Nagoya464-8650, Japan4
Department of Medical Microbiology, Division of Molecular Pathology Infection and Immunity, St Bartholomew’s and the Royal London School of Medicine and Dentistry, London E1 2AA, UK5
Department of Microbiology, School of Dentistry, Nagasaki University, Nagasaki 852-8588, Japan6

Author for correspondence: Koji Nakayama. Tel: +81 95 849 7648. Fax: +81 95 849 7650. e-mail: knak{at}net.nagasaki-u.ac.jp

A nonpigmented mutant of Porphyromonas gingivalis was constructed by using transposon mutagenesis. The mutant possessed the transposon DNA at the novel gene porR. Gene targeted mutagenesis revealed that porR was responsible for pigmentation. The porR gene shared similarities with genes of the degT family, the products of which are now considered to be transaminases involved in biosynthesis of sugar portions of cell-surface polysaccharides and aminoglycosides. The porR mutant showed a pleiotropic phenotype: delayed maturation of fimbrillin, preferential presence of Rgp and Kgp proteinases in culture supernatants, and no haemagglutination. The porR mutant had altered phenol extractable polysaccharide compared to the porR+ sibling strain. A mAb, 1B5, that reacts with sugar portions of P. gingivalis cell surface polysaccharide and membrane-type Rgp proteinase showed no reaction with the cell lysates of the porR mutant. These results indicate that porR is involved in biosynthesis of cell surface polysaccharide that may function as an anchorage for Rgp, Kgp, haemagglutinins and the haemoglobin receptor protein.

Keywords: cell surface polysaccharide, cysteine proteinases, colonial pigmentation, haemagglutination, fimbrillin maturation

Abbreviations: HA, haemagglutinin; HbR, haemoglobin receptor protein; Kgp, Lys-gingipain; mt, membrane type; Rgp, Arg-gingipain; TLCK, N{alpha}-p-tosyl-L-lysine chloromethyl ketone

The GenBank/EMBL/DDBJ accession number for the sequences reported in this paper is D64132.

a M.S. and D.B.R. contributed equally to this work.

b Present address: Department of Microbiology, School of Dentistry, Nagasaki University, Nagasaki 852-8588, Japan.




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