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Difference in substrate specificity divides the yeast alkali-metal-cation/H⁺ antiporters into two subfamilies

Department of Membrane Transport, Institute of Physiology CzAcadSci, 14220 Prague 4, Czech Republic¹
Laboratory of Microbiology and Genetics, UPRES A-7010-CNRS, Université Louis Pasteur, 67083 Strasbourg, France²

Author for correspondence: Hana Sychrová. Tel: +420 2 475 26 67. Fax: +420 2 475 24 88. e-mail: sychrova{at}biomed.cas.cz

Yeast plasma membrane Na⁺/H⁺ antiporters (TC 2.A.36) share a high degree of similarity at the protein level. Expression of four antiporters (Saccharomyces cerevisiae Nha1p, Candida albicans Cnh1p, Zygosaccharomyces rouxii ZrSod2-22p and Schizosaccharomyces pombe sod2p) in a Sacch. cerevisiae mutant strain lacking both Na⁺-ATPase and Na⁺/H⁺ antiporter genes made it possible to study the transport properties and contribution to cell salt tolerance of all antiporters under the same conditions. The ZrSod2-22p of the osmotolerant yeast Z. rouxii has the highest transport capacity for lithium and sodium but, like the Schiz. pombe sod2p, it does not recognize K⁺ and Rb⁺ as substrates. The Sacch. cerevisiae Nha1p and C. albicans Cnh1p have a broad substrate specificity for at least four alkali metal cations (Na⁺, Li⁺, K⁺, Rb⁺), but their contribution to overall cell tolerance to high external concentration of toxic Na⁺ and Li⁺ cations seems to be lower compared to the antiporters of Schiz. pombe and especially Z. rouxii.

Keywords: Na⁺ efflux, K⁺ efflux, salt tolerance, transport

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