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Research Paper |
National Food Biotechnology Centre1, Department of Microbiology2, Department of Medicine4, University College Cork, Cork, Ireland
Laboratory of Microbial Gene Technology, Agricultural University of Norway, N-1432 
s, Norway3
Author for correspondence: Douwe van Sinderen. Tel: +353 21 4902811. Fax: +353 21 4903101. e-mail: d.vansinderen{at}ucc.ie
ABP-118, a small heat-stable bacteriocin produced by Lactobacillus salivarius subsp. salivarius UCC118, a strain isolated from the ileal–caecal region of the human gastrointestinal tract, was purified to homogeneity. Using reverse genetics, a DNA fragment specifying part of ABP-118 was identified on a 10769 bp chromosomal region. Analysis of this region revealed that ABP-118 was a Class IIb two-peptide bacteriocin composed of Abp118
, which exhibited the antimicrobial activity, and Abp118ß, which enhanced the antimicrobial activity. The gene conferring strain UCC118 immunity to the action of ABP-118, abpIM, was identified downstream of the abp118ß gene. Located further downstream of abp118ß, several ORFs were identified whose deduced proteins resembled those of proteins involved in bacteriocin regulation and secretion. Heterologous expression of ABP-118 was achieved in Lactobacillus plantarum, Lactococcus lactis and Bacillus cereus. In addition, the abp118 locus encoded an inducing peptide, AbpIP, which was shown to play a role in the regulation of ABP-118 production. This novel bacteriocin is, to the authors’ knowledge, the first to be isolated from a known human probiotic bacterium and to be characterized at the genetic level.
Keywords: lactic acid bacteria, two-peptide bacteriocin, bacterial immunity
Abbreviations: ABC, ATP-binding cassette; AbpIP, induction factor; AU, activity unit; Bac, bacteriocin production; CFS, cell-free supernatant; LAB, lactic acid bacteria; Lactobacillus salivarius, Lactobacillus salivarius subsp. salivarius
The GenBank accession number for the sequence reported in this paper is AF408405.
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