Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bralley, P.
Right arrow Articles by Jones, G. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bralley, P.
Right arrow Articles by Jones, G. H.
Agricola
Right arrow Articles by Bralley, P.
Right arrow Articles by Jones, G. H.
Microbiology (2002), 148, 1421-1425.
© 2002 Society for General Microbiology

Research Paper

cDNA cloning confirms the polyadenylation of RNA decay intermediates in Streptomyces coelicolor

Patricia Bralley1 and George H. Jones1

Department of Biology, Emory University, Atlanta, GA 30322, USA1

Author for correspondence: Patricia Bralley. Tel: +1 404 727 4208. Fax: +1 404 727 2880. e-mail: pbralley{at}biology.emory.edu

In Escherichia coli the poly(A) tails of messenger and rRNAs are a major determinant of RNA stability. These tails are formed primarily by poly(A) polymerase I (PAP I) in wild-type strains or by polynucleotide phosphorylase (PNPase) in PAP I-deficient strains. In Streptomyces coelicolor it has been shown that mycelial RNAs display biochemical characteristics consistent with the presence of poly(A) tails. To confirm the occurrence of polyadenylation, rRNA and mRNA transcripts from S. coelicolor were isolated by oligo(dT)-dependent RT-PCR followed by cDNA cloning. One of the clones obtained was polyadenylated at a site corresponding to the mature 3' terminus of 16S rRNA, while two 23S rRNA cDNA clones were polyadenylated at precursor processing sites. Other clones identified polyadenylation sites internal to the coding regions of both 16S and 23S rRNAs, and redD and actII-orf4 mRNAs. While most rRNA cDNA clones displayed adenosine homopolymer tails, the poly(A) tails of three rRNAs and all the redD and actII-orf4 clones consisted of a variety of heteropolymers. These results suggest that the enzyme primarily responsible for polyadenylation in S. coelicolor is PNPase rather than a PAP I homologue.

Keywords: redD, actII-orf4, polynucleotide phosphorylase, poly(A) polymerase

Abbreviations: PAP I, poly(A) polymerase I; PNPase, polynucleotide phosphorylase




This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
S. A. Chang, M. Cozad, G. A. Mackie, and G. H. Jones
Kinetics of Polynucleotide Phosphorylase: Comparison of Enzymes from Streptomyces and Escherichia coli and Effects of Nucleoside Diphosphates
J. Bacteriol., January 1, 2008; 190(1): 98 - 106.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. Portnoy and G. Schuster
RNA polyadenylation and degradation in different Archaea; roles of the exosome and RNase R
Nucleic Acids Res., November 6, 2006; 34(20): 5923 - 5931.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Slomovic, D. Laufer, D. Geiger, and G. Schuster
Polyadenylation of ribosomal RNA in human cells
Nucleic Acids Res., May 31, 2006; 34(10): 2966 - 2975.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
P. Bralley, B. Gust, S. Chang, K. F. Chater, and G. H. Jones
RNA 3'-tail synthesis in Streptomyces: in vitro and in vivo activities of RNase PH, the SCO3896 gene product and polynucleotide phosphorylase.
Microbiology, March 1, 2006; 152(Pt 3): 627 - 636.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
P. Bralley, S. A. Chang, and G. H. Jones
A Phylogeny of Bacterial RNA Nucleotidyltransferases: Bacillus halodurans Contains Two tRNA Nucleotidyltransferases
J. Bacteriol., September 1, 2005; 187(17): 5927 - 5936.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
J. Campos-Guillen, P. Bralley, G. H. Jones, D. H. Bechhofer, and G. Olmedo-Alvarez
Addition of Poly(A) and Heteropolymeric 3' Ends in Bacillus subtilis Wild-Type and Polynucleotide Phosphorylase-Deficient Strains
J. Bacteriol., July 15, 2005; 187(14): 4698 - 4706.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
P. Bralley and G. H. Jones
Organization and Expression of the Polynucleotide Phosphorylase Gene (pnp) of Streptomyces: Processing of pnp Transcripts in Streptomyces antibioticus
J. Bacteriol., May 15, 2004; 186(10): 3160 - 3172.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
B. Sohlberg, J. Huang, and S. N. Cohen
The Streptomyces coelicolor Polynucleotide Phosphorylase Homologue, and Not the Putative Poly(A) Polymerase, Can Polyadenylate RNA
J. Bacteriol., December 15, 2003; 185(24): 7273 - 7278.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
P. Bralley and G. H. Jones
Overexpression of the polynucleotide phosphorylase gene (pnp) of Streptomyces antibioticus affects mRNA stability and poly(A) tail length but not ppGpp levels
Microbiology, August 1, 2003; 149(8): 2173 - 2182.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Rott, G. Zipor, V. Portnoy, V. Liveanu, and G. Schuster
RNA Polyadenylation and Degradation in Cyanobacteria Are Similar to the Chloroplast but Different from Escherichia coli
J. Biol. Chem., April 25, 2003; 278(18): 15771 - 15777.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2002 Society for General Microbiology.